Anti-BrdU antibody [IIB5]

• 产品名称

  Anti-BrdU抗体[IIB5]
  参阅全部 BrdU 一抗

• 描述

  小鼠单克隆抗体[IIB5] to BrdU

• 宿主

  Mouse

• 特异性

  This antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU).

• 经测试应用

  适用于: ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Chemical/ Small Molecule corresponding to BrdU conjugated to bovine serum albumin.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  IIB5

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab8955于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 细胞定位

  Nuclear

• 别名

• Bromodeoxyuridine antibody

• BUdr antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)

  Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)

  BrdU staining in sponge Halisarca caerulea using ab8955. Metacrylate embedded tissue sections were incubated with 1:200 diluted primary antibody. An avidin-biotin complex kit using biotinylated rabbit anti-mouse antibody and then incubation in avidin-biotin-peroxidase complex was used for detection. 

• 

  Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [IIB5] (ab8955)

  Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells using ab8955 (1:1000).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (ab8955)

  BrdU staining in paraffin embedded rat intestine using ab8955. Primary antibody diluted 1:200.  Peroxidase-conjugated goat anti mouse secondary antibody was used for detection.

• 

  Flow Cytometry - Anti-BrdU antibody [IIB5] (ab8955)

  Flow cytometric BrdU-DNA cell cycle analysis in a lung cancer cell line. Dilution 1:100 of primary antibody ab8955. FITC-conjugated goat anti mouse secondary antibody was used for detection.