Anti-PPAR delta抗体
参阅全部 PPAR delta 一抗
兔多克隆抗体to PPAR delta
Rabbit
This affinity purified antibody is directed against mouse PPAR delta protein. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
适用于: ICC/IF, WB, ELISA, IHC-Pmore details
与反应: Mouse, Human
预测可用于: Rat
Synthetic peptide:
MEQPQEETPEAREE
, corresponding to N terminal amino acids 1-14 of Mouse PPAR delta .
3T3 cell lysate HepG2
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1.57 mg/ml
Immunogen affinity purified
This affinity purified antibody is directed against mouse PPAR delta protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
多克隆
IgG
Abpromise™承诺保证使用ab8937于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | (3) | 1/500 - 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 50 kDa). |
| ELISA | 1/30000 - 1/70000. | |
| IHC-P | 1/500. |
Entrez Gene: 5467 Human
Entrez Gene: 19015 Mouse
Omim: 600409 Human
SwissProt: Q03181 Human
SwissProt: P35396 Mouse
Unigene: 696032 Human
Unigene: 328914 Mouse
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Immunocytochemistry/ Immunofluorescence - Anti-PPAR delta antibody (ab8937)
ICC/IF image of ab8937 stained HepG2 cells (ab7900). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8937, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-PPAR delta antibody (ab8937)
Rabbit polyclonal to PPAR delta (ab8937).
Each lane contains 20 ug 3T3 cell lysate
Lane 1: ab8937 1/500
Lane 2: ab8937 1/1000
Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000
Rabbit polyclonal to PPAR delta (ab8937). Each lane contains 20 ug 3T3 cell lysate (ab7179) Lane 1: ab8937 1/500 Lane 2: ab8937 1/1000 Secondary antibody: Goat anti-Rabbit (HRP) (ab6721) at 1/2000
