Anti-pan-AKT抗体
参阅全部 pan-AKT 一抗
兔多克隆抗体to pan-AKT
Rabbit
Pan Anti-AKT Antibody reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
适用于: IHC-P, WB, ICC/IFmore details
与反应: Mouse, Rat, Human
预测可用于: Chicken
Synthetic peptide:
CRPHFPQFSYSASGTA
conjugated to KLH, corresponding to amino acids 466-480 of Human pan-AKT (100% similar to Rat, Chicken and Mouse AKT sequences). (Peptide available as ab9041.)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride, 98.6% Whole serum
0.02M Potassium phosphate, 0.15M Sodium chloride
Whole antiserum
This product was prepared from monospecific antiserum by a delipidation and defibrination.
多克隆
IgG
Abpromise™承诺保证使用ab8805于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) | 1/500 - 1/2000. |
| WB | (9) | 1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa). |
| ICC/IF | (1) | 1/100 - 1/1000. |
Entrez Gene: 207 Human
Entrez Gene: 11651 Mouse
Omim: 164730 Human
SwissProt: O57513 Chicken
SwissProt: P31749 Human
SwissProt: P31750 Mouse
Unigene: 525622 Human
Unigene: 6645 Mouse
Unigene: 11422 Rat
AKT antibody
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AKT1_HUMAN antibody
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PRKBA antibody
PRKBB antibody
PRKBG antibody
Protein kinase B antibody
Protein kinase B beta antibody
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RAC alpha antibody
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RAC gamma antibody
RAC PK alpha antibody
RAC PK beta antibody
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RAC-alpha serine/threonine-protein kinase antibody
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STK2 antibody
Western blot - Anti-pan-AKT antibody (ab8805)
Western blot using Akt antibody ab8805 at 1/500, Goat anti-rabbit IgG peroxidase conjugate at 1/10,000. 20 ug NIH/3T3 whole cell lysate, Gel type 10% NuPage with MOPS buffer developed with Substrate Pierce SuperSignal™ West Pico.
Immunocytochemistry/ Immunofluorescence - Anti-pan-AKT antibody (ab8805)This image is courtesy of Mark Sussman, Cincinnati, USA
The Akt antibody (ab8805) is used at 1/80 on cultured neonatal rat cardiomyocytes that express a nuclear-targeted Akt construct. The green is Akt antibody, the red is Texas-red™ phalloidin that labels actin filaments.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan-AKT antibody (ab8805)
A: Normal colon tissue
B: Tumour tissue
Akt antibody (ab8805) used at 1/1000 on formalin-fixed paraffin embedded tissue.
Anti-pan-AKT antibody (ab8805)
Western blot - Anti-pan-AKT antibody (ab8805)This image is a courtesy of Anonymous Abreview
Anti-pan-AKT antibody (ab8805) at 1/500 dilution + Lysate prepared from human HT1080 cell line at 10 µg
Secondary
HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 57 kDawhy is the actual band size different from the predicted?
Exposure time: 6 minutes
