Anti-CD34 antibody [QBEND-10]

• 产品名称

  Anti-CD34抗体[QBEND-10]
  参阅全部 CD34 一抗

• 描述

  小鼠单克隆抗体[QBEND/10] to CD34

• 宿主

  Mouse

• 特异性

  This antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immuno-gold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A.

• 经测试应用

  适用于: Flow Cyt, IHC-Pmore details
  不适用于: WB

• 种属反应性

  与反应: Human
  不与反应: Rat, Sheep, Cow, Dog

• 免疫原

  Tissue, cells or virus corresponding to Human CD34. Human endothelial vesicles
  Database link: P28906

• 表位

  This antibody's target epitope is distinct from those of other anti-CD34 reactive mabs as demonstrated by its lack of sensitivity to neuraminidase treatment, and fluorescence staining profiles during flow cytometry analysis of target cell populations.

• 阳性对照

• tonsil, bone marrow

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: PBS

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  QBEND/10

• 骨髓瘤

  NS0

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab8536于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for WB.

• 数据库链接

• Entrez Gene: 947 Human

• Omim: 142230 Human

• SwissProt: P28906 Human

• Unigene: 374990 Human

• 别名

• CD34_HUMAN antibody

• Cluster designation 34 antibody

• cluster of differentiation 34 antibody

• Hematopoietic progenitor cell antigen CD34 antibody

• HPCA1 antibody

• Mucosialin antibody

• OTTHUMP00000034733 antibody

• OTTHUMP00000034734 antibody

• CD34 antibody

• CD34 antigen antibody

• CD34 molecule antibody

• 

  Flow Cytometry - Anti-CD34 antibody [QBEND-10] (ab8536)

  Overlay histogram showing Jurkat cells stained with ab8536 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8536, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
  
  Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.

  Detection system polymeric HRP. HIER with Pressure cooker pH6.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an Abreview by Carl Hobbs.

  ab8536 staining CD34 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.

  Detection system polymeric HRP. HIER with Pressure cooker pH6.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD34 with ab8536 at 1ug/ml.
  Detection system polymeric HRP.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an abreview submitted by Manoj Kumar Valluru.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human umbilical cord labeling CD34 with ab8536 at 1/1000 dilution. Tissue was fixed in formaldehyde and permeabilized with PBS. Heat mediated antigen retrieval was performed using a citrate pH 6 buffer. Tissue was blocked with 5% serum for 1 hour at room temperature. A biotinylated polyclonal goat anti-mouse secondary antibody was used at 1/100 dilution.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an Abreview submitted by Dr Jose Javier Martin De Llano

  ab8536 at 1/500 staining human umbilical cord tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with horse serum before incubation with the antibody for 16 hours. A biotinylated goat anti-mouse IgG was used as the secondary.

  See Abreview