Anti-BNIP3L/NIX抗体
参阅全部 BNIP3L/NIX 一抗
兔多克隆抗体to BNIP3L/NIX
Rabbit
适用于: WB, ICC/IF, IHC-Pmore details
与反应: Human
预测可用于: Mouse, Rat
Synthetic peptide corresponding to Human BNIP3L/NIX aa 77-92.
Sequence:
DAQHESGQSSSRGSSH
K562 cell lysatecan be used as positive control and the approximate 40kDa top band represents monomer of Bnip3L/NIX (4).
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L/NIX, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L/NIX induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L/NIX interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L/NIX is localized in mitochondria. The messenger RNA of Bnip3L/NIX is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L/NIX and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C.
pH: 7.2
Preservative: 0.02% Sodium azide
Affinity purified
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growingsubclass of the Bcl-2 family. A novel BH3 domaincontaining protein was recently identified and designated Bnip3L, Bnip3a, and Nix (for Nip3-like protein X) (Matsushima, M. et al.; Yasuda, M. et al.; Chen, G, et al.).Bnip3L/Bnip3a/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Over expression of Bnip3L induces apoptosis (Yasuda, M. et al.; Chen, G, et al.). Bnip3L interacts withand overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3Lis localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues (Matsushima, M. et al.; Yasuda, M. et al.). Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
多克隆
IgG
unknown
Abpromise™承诺保证使用ab8399于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa. To reduce possible background load less sample and or use more diluted antibody. | |
| ICC/IF | (1) | Use at an assay dependent concentration. See Abreview. |
| IHC-P | Use a concentration of 2 µg/ml. |
Entrez Gene: 665 Human
Entrez Gene: 12177 Mouse
Omim: 605368 Human
SwissProt: O60238 Human
SwissProt: Q9Z2F7 Mouse
Unigene: 131226 Human
Unigene: 29820 Mouse
Unigene: 347081 Mouse
Adenovirus E1B19k binding protein B5 antibody
Adenovirus E1B19K-binding protein B5 antibody
BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
BCL2/adenovirus E1B 19 kDa protein interacting protein 3A antibody
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like antibody
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A antibody
BCL2/adenovirus E1B 19kDa interacting protein 3 like antibody
BNI3L_HUMAN antibody
BNIP3a antibody
BNIP3H antibody
BNIP3L antibody
BNIP3L protein antibody
NIP3 like protein X antibody
NIP3-like protein X antibody
NIP3L antibody
NIX antibody
Western blot - Anti-BNIP3L/NIX antibody (ab8399)
All lanes : Anti-BNIP3L/NIX antibody (ab8399) at 1 µg/ml
Lane 1 : K562 whole cell lysate with absence of immunogenic peptide
Lane 2 : K562 whole cell lysate with presence of immunogenic peptide
The approximate 40 kDa top band represents monomer of Bnip3L/NIX.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BNIP3L/NIX antibody (ab8399)
ab8399 at 2µg/ml staining BNIP3L/NIX in human kidney tissue by IHC
Immunocytochemistry/ Immunofluorescence - Anti-BNIP3L/NIX antibody (ab8399)
Immunofluorescence of Bnip3L/NIX in Human Kidney cells using ab8399 at 10 ug/ml.
