Anti-ATP1B1 antibody [464.8]

• 产品名称

  Anti-ATP1B1抗体[464.8]
  参阅全部 ATP1B1 一抗

• 描述

  小鼠单克隆抗体[464.8] to ATP1B1

• 宿主

  Mouse

• 经测试应用

  适用于: WB, Flow Cyt (Intra)more details

• 种属反应性

  与反应: Human
  预测可用于: Rat, Cow, Dog, Pig

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: Human kidney tissue lysates Flow Cyt (Intra): HepG2 cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituent: PBS
  
  Contains 0.4M arginine

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  464.8

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab8344于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 403966 Dog

• Entrez Gene: 481 Human

• Entrez Gene: 396898 Pig

• Entrez Gene: 25650 Rat

• Omim: 182330 Human

• SwissProt: P06583 Dog

• SwissProt: P05026 Human

• SwissProt: P05027 Pig

• SwissProt: P07340 Rat

• Unigene: 291196 Human

• Unigene: 8925 Rat

别名

• Adenosinetriphosphatase antibody

• AT1B1_HUMAN antibody

• ATP 1B antibody

• ATP1B antibody

• ATP1B1 antibody

• ATPase Na+/K+ transporting beta 1 polypeptide antibody

• Atpb 1 antibody

• ATPBS antibody

• Beta 1 subunit of Na(+) K(+) ATPase antibody

• Na K ATPase beta 1 polypeptide antibody

• Na+/K+ ATPase beta 1 subunit antibody

• Sodium/potassium dependent ATPase beta 1 subunit antibody

• Sodium/potassium transporting ATPase beta 1 chain antibody

• Sodium/potassium transporting ATPase subunit beta 1 antibody

• Sodium/potassium-dependent ATPase subunit beta-1 antibody

• Sodium/potassium-transporting ATPase subunit beta-1 antibody

• 

  Western blot - Anti-ATP1B1 antibody [464.8] (ab8344)

  Anti-ATP1B1 antibody [464.8] (ab8344) at 1/500 dilution + Human kidney tissue lysate - total protein (ab30203) at 20 µg
  
  Secondary
  Granzyme B overexpression 293T lysate (whole cell) (ab94040) at 1/10000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 35 kDa
  Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 4 minutes
  

• 

  Flow Cytometry (Intracellular) - Anti-ATP1B1 antibody [464.8] (ab8344)

  Overlay histogram showing HepG2 cells stained with ab8344 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8344, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.