Anti-TLR4 antibody [HTA125]

• 产品名称

  Anti-TLR4抗体[HTA125]
  参阅全部 TLR4 一抗

• 描述

  小鼠单克隆抗体[HTA125] to TLR4

• 宿主

  Mouse

• 特异性

  This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 has been demonstrated to act as alpha receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. We do not guarantee WB since we receive variable results from our customers and cannot make this antibody work in our hands in WB in denaturing and reducing conditions. The immunogen is a full-length immunogen so we suspect that to get the product to work in WB, non-reducing or native WB could be a better option. THP1 monocytes or macrophage are good positive controls and HeLa/Hek293 should be suitable negative/low expression controls. Membrane preparation may be better that whole cell as expression levels may not be that high in some samples. We welcome any further feedback in WB by participating to our AbTrial program.

• 经测试应用

  适用于: IP, Flow Cytmore details

• 种属反应性

  与反应: Rat, Guinea pig, Human, Pig, Rhesus monkey
  

• 免疫原

  Tissue, cells or virus corresponding to TLR4. Ba/F3 cell line expressing TLR4

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.09% Sodium azide
  Constituent: PBS

• 纯度

  Protein G purified

• 克隆

  单克隆

• 克隆编号

  HTA125

• 骨髓瘤

  Sp2/0

• 同种型

  IgG2a

The Abpromise guarantee

Abpromise™承诺保证使用ab8376于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 7099 Human

• Entrez Gene: 399541 Pig

• Entrez Gene: 29260 Rat

• Omim: 603030 Human

• SwissProt: O00206 Human

• SwissProt: Q68Y56 Pig

• SwissProt: Q9QX05 Rat

• Unigene: 174312 Human

• Unigene: 14534 Rat

别名

• ARMD10 antibody

• CD284 antibody

• CD284 antigen antibody

• Homolog of Drosophila toll antibody

• hToll antibody

• TLR 4 antibody

• TLR4 antibody

• TLR4_HUMAN antibody

• TOLL antibody

• Toll like receptor 4 antibody

• Toll-like receptor 4 antibody

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  Flow Cytometry - Anti-TLR4 antibody [HTA125] (ab8376)

  Staining of human peripheral blood monocytes with ab8376.