FITC Anti-alpha smooth muscle Actin antibody [1A4]

• 产品名称

  FITC荧光Anti-alpha smooth muscle Actin抗体[1A4]
  参阅全部 alpha smooth muscle Actin 一抗

• 描述

  FITC荧光小鼠单克隆抗体[1A4] to alpha smooth muscle Actin

• 宿主

  Mouse

• 偶联物

  FITC. Ex: 493nm, Em: 528nm

• 经测试应用

  适用于: ICC/IF, WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Sheep, Goat, Chicken, Guinea pig, Cat, Dog, Snake, Zebrafish

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• ICC: SV40LT-SMC cells.

• 常规说明

  This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary. This antibody strongly crossreacts with alphasmooth muscle actin in a variety of mammalian and non-mammalian species.

  The antibody is conjugated with FITC at a ratio 6 Moles of Fluorescein to 1 Mole of antibody. Maximum excitation of FITC occurs at wavelength 492 nm, and maximum emission occurs at wavelenght 520 nm.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Store In the Dark.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA

• 纯度

  Affinity purified

• Primary antibody说明

  This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis and ovary. This antibody strongly crossreacts with alphasmooth muscle actin in a variety of mammalian and non-mammalian species.

• 克隆

  单克隆

• 克隆编号

  1A4

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab8211于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 101093547 Cat

• Entrez Gene: 423787 Chicken

• Entrez Gene: 477587 Dog

• Entrez Gene: 59 Human

• Entrez Gene: 11475 Mouse

• Entrez Gene: 81633 Rat

• Entrez Gene: 101121051 Sheep

• Entrez Gene: 322509 Zebrafish

• Omim: 102620 Human

• SwissProt: P08023 Chicken

• SwissProt: P62736 Human

• SwissProt: P62737 Mouse

• SwissProt: P62738 Rat

• Unigene: 500483 Human

• Unigene: 213025 Mouse

• Unigene: 195319 Rat

• Unigene: 3114 Rat

别名

• a actin antibody

• AAT6 antibody

• ACTA_HUMAN antibody

• ACTA2 antibody

• Actin alpha 2 smooth muscle aorta antibody

• Actin aortic smooth muscle antibody

• Actin, aortic smooth muscle antibody

• ACTSA antibody

• ACTVS antibody

• Alpha 2 actin antibody

• Alpha actin 2 antibody

• Alpha cardiac actin antibody

• alpha sma antibody

• Alpha-actin-2 antibody

• Cell growth inhibiting gene 46 protein antibody

• Cell growth-inhibiting gene 46 protein antibody

• GIG46 antibody

• Growth inhibiting gene 46 antibody

• MYMY5 antibody

• 

  Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)

  ab8211 staining alpha smooth muscle Actin in wild-type HeLa cells (top panel) and ACTA2 knockout HeLa cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab8211 at 5μg/ml concentration and ab195884 (Rat monoclonal to Tubulin - Alexa Fluor® 647) at 1/100 dilution overnight at 4°C. Nuclear DNA was labelled in blue with DAPI.
  Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• 

  Immunocytochemistry/ Immunofluorescence - FITC Anti-alpha smooth muscle Actin antibody [1A4] (ab8211)

  ab8211 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8211 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  This product also gave a positive signal under the same testing conditions in SV40LT-SMC cells fixed with 4% formaldehyde (10 min).