Anti-Collagen III antibody

• 产品名称

  Anti-Collagen III抗体
  参阅全部 Collagen III 一抗

• 描述

  兔多克隆抗体to Collagen III

• 宿主

  Rabbit

• 特异性

  This antibody reacts with most mammalian Type III collagens and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

• 经测试应用

  适用于: ICC/IF, IP, ELISA, IHC-P, WBmore details

• 种属反应性

  与反应: Rat, Cow, Human
  预测可用于: Mammals

• 免疫原

  Full length native protein (purified) corresponding to Collagen III aa 1-1466.
  Database link: P02461

• 阳性对照

• Natural Cow Collagen III protein (ab7528) can be used as a positive control in WB. Human skin. Human testicle tissue.

• 常规说明

  For more protocol tips, please see: https://www.abcam.com/protocols/collagen

  At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 浓度

• 批次浓度范围 100 µg 浓度为 1 - 1.16 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Collagen III Antibody has been prepared by immunoaffinity chromatography using immobilized antigens.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab7778于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

  hide

• Unigene: 3247 Rat

• Entrez Gene: 510833 Cow

• Entrez Gene: 1281 Human

• Entrez Gene: 84032 Rat

• Omim: 120180 Human

• SwissProt: P04258 Cow

• SwissProt: P02461 Human

• SwissProt: P13941 Rat

• Unigene: 443625 Human

• 别名

• COL 3A1 antibody

• COL3A1 antibody

• Collagen alpha 1(III) chain antibody

• Collagen alpha-1(III) chain antibody

• Collagen III alpha 1 chain precursor antibody

• Collagen III alpha 1 polypeptide antibody

• Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant) antibody

• Collagen type III alpha 1 antibody

• Collagen type III alpha 1 chain antibody

• Collagen type III alpha antibody

• Collagen, fetal antibody

• EDS4A antibody

• Ehlers Danlos syndrome type IV, autosomal dominant antibody

• Fetal collagen antibody

• Type III collagen antibody

• Alpha 1 type III collagen antibody

• Alpha1 (III) collagen antibody

• CO3A1_HUMAN antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)Image courtesy of Rudolf Jung by Abreview.

  ab7778 staining Collagen III in human testicle tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using TE buffer pH 9.0. Samples were then incubated with ab7778 at a 1/200 dilution for 30 minutes at 20°C. The secondary used was an undiluted, HRP-conjugated goat anti-rabbit polyclonal.

  See Abreview

• 

  Western blot - Anti-Collagen III antibody (ab7778)

  Anti-Collagen III antibody (ab7778) at 1/1000 dilution + Human collagen III at 0.1 µg
  
  Predicted band size: 138 kDa
  
  
  
  Western Blot produced under denaturing and reducing conditions.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

  ab7778 at 1:400 (45 min RT) showed strong staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 (ab64214) at 99-100°C - 20 minutes for antigen retrieval.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

  Immunohistochemistry of ab7778. Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4°C for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3’-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (ab7778)

  Ab53088 staining Human skin (ab30166). Staining is localised to the extracellular matrix.
  Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer citrate pH 6.0 (ab64214) in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.