Anti-CD45RA抗体[MEM-56]
参阅全部 CD45RA 一抗
小鼠单克隆抗体[MEM-56] to CD45RA
Mouse
适用于: WB, IHC-P, Flow Cytmore details
与反应: Human
Tissue, cells or virus corresponding to Human CD45RA. Thymocytes and T lymphocytes
Database link: P08575-8
WB: HUT-78 human cutaneous T cell lymphoma cell lysate.
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
Purified from TCS
单克隆
MEM-56
IgG2b
Abpromise™承诺保证使用ab8217于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | Use at an assay dependent concentration. Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs. | |
IHC-P | Use at an assay dependent concentration. | |
Flow Cyt | Use a concentration of 0.3 - 4 µg/ml. |
Entrez Gene: 5788 Human
Omim: 151460 Human
SwissProt: P08575 Human
Unigene: 654514 Human
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Flow Cytometry - Anti-CD45RA antibody [MEM-56] (ab8217)
Flow cytometric analysis of Human Peripheral Blood cells labelling CD45RA with ab8217 at 0.3 ug/ml showing separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [MEM-56] (ab8217)
Immunohistochemical analysis of Paraffin-embedded sections Human Tonsil tissue labelling CD45RA with ab8217 at 5µg/ml.
Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)
Lane 1 : CD45 control antibody
Lanes 2-3 : Anti-CD45RA antibody [MEM-56] (ab8217)
Lane 1 : HUT-78 human cutaneous T cell lymphoma cell line.
Lane 2 : sample of cell lysate prepared under non-reducing conditions
Lane 3 : sample of cell lysate prepared under reducing conditions