Anti-68kDa Neurofilament/NF-L antibody [DA2]

• 产品名称

  Anti-68kDa Neurofilament/NF-L抗体[DA2]
  参阅全部 68kDa Neurofilament/NF-L 一抗

• 描述

  小鼠单克隆抗体[DA2] to 68kDa Neurofilament/NF-L

• 宿主

  Mouse

• 特异性

  Specifically recognizes the light neurofilament subunit or 68kDa Neurofilament/NF-L.

• 经测试应用

  适用于: Flow Cyt, ICC/IF, WB, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  预测可用于: Bird, Mammals

• 免疫原

  Full length native protein (purified) corresponding to Pig 68kDa Neurofilament/NF-L. A preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H. Screening was by ELISA on the immunogen followed by immunofluorescence microscopy.

• 表位

  Epitope mapped to a short peptide in the C-terminal “tail” region of the molecule within the human sequence SYYTSHVQEEQIEVE, amino acids 441-455.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.033% Sodium azide
  Constituent: Tissue culture supernatant

• 纯度

  Tissue culture supernatant

• 克隆

  单克隆

• 克隆编号

  DA2

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab7255于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 4747 Human

• Entrez Gene: 18039 Mouse

• Entrez Gene: 83613 Rat

• Omim: 162280 Human

• SwissProt: P07196 Human

• SwissProt: P08551 Mouse

• SwissProt: P19527 Rat

• Unigene: 521461 Human

• Unigene: 1956 Mouse

• Unigene: 18568 Rat

别名

• 68 kDa neurofilament protein antibody

• 68kDa Neurofilament antibody

• 68kDa neurofilament protein antibody

• CMT1F antibody

• CMT2E antibody

• FLJ53642 antibody

• Light molecular weight neurofilament protein antibody

• NEFL antibody

• Neurofilament light antibody

• Neurofilament light polypeptide 68kDa antibody

• Neurofilament light polypeptide antibody

• Neurofilament protein, light chain antibody

• Neurofilament subunit NF L antibody

• Neurofilament triplet L protein antibody

• NF-L antibody

• NF68 antibody

• NFL antibody

• NFL_HUMAN antibody

• 

  Western blot - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)

  All lanes : Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255) at 1/5000 dilution
  
  Lane 1 : rat brain whole tissue lysates
  Lane 2 : rat spinal cord whole tissue lysates
  Lane 3 : mouse brain whole tissue lysates
  Lane 4 : mouse spinal cord whole tissue lysates
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)

  IHC image of ab7255 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7255, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)

  Ab7255 staining 68kDa Neurofilament/NF-L in rat frontal cortex sections by Immunocytochemistry/Immunofluorescence.  Samples were incubated with primary antibody at 1/5000 dilution (red) and chicken polyclonal to GFAP. 

• 

  Western blot - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)

  Lane 1 : Coomassie Blue stain
  Lane 2 : Heavy neurofilament antibody
  Lane 3 : Anti-160 kD Neurofilament Medium antibody [3H11] (ab7256)
  Lane 4 : Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)
  Lane 5 : alpha Internexin antibody
  Lane 6 : GFAP antibody
  
  All lanes : Cytoskeletal homogenates of rat spinal cord
  

• 

  Flow Cytometry - Anti-68kDa Neurofilament/NF-L antibody [DA2] (ab7255)

  Overlay histogram showing SH-SY5Y cells stained with ab7255 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7255, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.