Anti-NFkB Inducing Kinase NIK抗体
参阅全部 NFkB Inducing Kinase NIK 一抗
兔多克隆抗体to NFkB Inducing Kinase NIK
Rabbit
适用于: ICC/IF, WBmore details
与反应: Human
Recombinant fragment corresponding to Human NFkB Inducing Kinase NIK aa 795-947.
Database link: Q99558
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS
Protein A purified
多克隆
IgG
Abpromise™承诺保证使用ab7204于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | (2) | 1/2000. |
Entrez Gene: 9020 Human
Omim: 604655 Human
SwissProt: Q99558 Human
Unigene: 404183 Human
Unigene: 668927 Human
Unigene: 735695 Human
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Western blot - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
All lanes : Anti-NFkB Inducing Kinase NIK antibody (ab7204)
Lane 1 : macrophages lysate
Lane 2 : NIK transfected cell lysate
Western blot detection of NIK in 1, macrophage and 2, NIK transfected cells by anti-human NIK.
Immunocytochemistry/ Immunofluorescence - Anti-NFkB Inducing Kinase NIK antibody (ab7204)
ICC/IF image of ab7204 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7204, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
