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Anti-liver FABP antibody [L2B10]

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50ug
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产品详情
  • 产品名称

    Anti-liver FABP抗体[L2B10]
    参阅全部 liver FABP 一抗

  • 描述

    小鼠单克隆抗体[L2B10] to liver FABP

  • 宿主

    Mouse

  • 特异性

    This monoclonal antibody binds to human liver FABP of both natural and recombinant origin. No reactivity to human intestinal or heart FABP.

  • 经测试应用

    适用于: WBFunctional StudiesFlow CytIHC-PIHC-Frmore details
    不适用于: ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Cow, Human, African green monkey

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA

  • 浓度

    • 50 µg 浓度为 0.1 mg/ml

  • 纯度

    Protein G purified

  • 克隆

    单克隆

  • 克隆编号

    L2B10

  • 同种型

    IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab7366于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
WB(6)

Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 18779054

Functional Studies

Use at an assay dependent concentration. 

Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant.

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Flow Cyt

Use 1µg for 106 cells. 

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P

Use at an assay dependent concentration.

IHC-Fr

Use at an assay dependent concentration.

应用说明

Is unsuitable for ICC/IF.

数据库链接

别名

  • FABP 1 antibody

  • FABP1 antibody

  • FABPL antibody

  • FABPL_HUMAN antibody

  • Fatty Acid Binding Protein 1 antibody

  • Fatty acid binding protein 1 liver antibody

  • Fatty Acid Binding Protein antibody

  • Fatty acid-binding protein 1 antibody

  • Fatty acid-binding protein antibody

  • Fatty acid-binding protein liver antibody

  • L FABP antibody

  • L-FABP antibody

  • liver antibody

  • Liver-type fatty acid-binding protein antibody

  • Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)

    Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)

    Immunohistochemical analysis of human L-FABP in frozen human kidney tissue with ab7366 at 1.9 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)

    Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 200X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)

    Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 50X.

  • Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)

    Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)

    Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


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