Anti-liver FABP抗体[L2B10]
参阅全部 liver FABP 一抗
小鼠单克隆抗体[L2B10] to liver FABP
Mouse
This monoclonal antibody binds to human liver FABP of both natural and recombinant origin. No reactivity to human intestinal or heart FABP.
适用于: WB, Functional Studies, Flow Cyt, IHC-P, IHC-Frmore details
不适用于: ICC/IF
与反应: Mouse, Rat, Cow, Human, African green monkey
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituent: 0.2% BSA
浓度
50 µg 浓度为 0.1 mg/ml
Protein G purified
单克隆
L2B10
IgG1
Abpromise™承诺保证使用ab7366于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (6) | Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 18779054 |
Functional Studies | Use at an assay dependent concentration. Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant. Read More | |
Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. | |
IHC-P | Use at an assay dependent concentration. | |
IHC-Fr | Use at an assay dependent concentration. |
应用说明
Is unsuitable for ICC/IF.
Entrez Gene: 2168 Human
Entrez Gene: 14080 Mouse
Omim: 134650 Human
SwissProt: P07148 Human
SwissProt: P12710 Mouse
Unigene: 380135 Human
Unigene: 22126 Mouse
Unigene: 36412 Rat
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Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)
Immunohistochemical analysis of human L-FABP in frozen human kidney tissue with ab7366 at 1.9 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 200X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 50X.
Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)
Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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