山羊F(ab)抗兔IgG H&L (Biotin)
参阅全部 IgG 二抗
Goat
Rabbit
No reaction was observed against anti-Papain or anti-Goat IgG F(c).
适用于: ICC/IF, Dot blot, WB, ELISA, IHC, Immunomicroscopymore details
Rabbit IgG whole molecule
Biotin
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG Fab molecule
多克隆
IgG
Abpromise™承诺保证使用ab7055于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | ||
| Dot blot | ||
| WB | ||
| ELISA | ||
| IHC | ||
| Immunomicroscopy |
应用说明
Dot: Use at an assay dependent dilution.
ELISA: 1/50000 - 1/250000.
IHC: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/50000 to 1/250000 of the reconstitution concentration is suggested for this product.
Suitable for other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat F(ab) Anti-Rabbit IgG H&L (Biotin) (ab7055)
ab7055 was used at dilution 1/200 with the primary antibody ab32090 in IHC-P. See the review on ab32090.
