山羊F(ab')2抗人IgG H&L (PE)预吸附二抗
参阅全部 IgG 二抗
Goat
Human
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), or Bovine, Horse, Mouse and Rat Serum Proteins.
适用于: Dot blot, ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
Cow, Horse, Mouse, Ratmore details
Full length native Human IgG (purified).
PE. Ex: 488nm, Em: 575nm
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
250 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8 Phycobiliprotein Concentration: 0.5 mg / ml (determined by absorbance = 82.0 at 565 nm for a 1% solution for only those R-PE molecules to which at least one molecule of active antibody is bound).
多克隆
IgG
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Abpromise™承诺保证使用ab7006于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Dot blot | Use at an assay dependent concentration. | |
ICC/IF | 1/100 - 1/250. | |
IHC-P | 1/50 - 1/200. | |
IHC-Fr | 1/50 - 1/200. | |
Immunomicroscopy | 1/50 - 1/200. | |
Flow Cyt | 1/100 - 1/250. Use 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. |