山羊抗小鼠IgG H&L (Alkaline Phosphatase)
参阅全部 IgG 二抗
Goat
Mouse
适用于: ICC/IF, Immunomicroscopy, WB, Dot blot, ELISA, IHC-P, IHC-Frmore details
mouse IgG whole molecule
Alkaline Phosphatase
Liquid
Shipped at 4°C. Store at +4°C.
pH: 8
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), 0.788% Tris HCl, 0.88% Sodium chloride, 0.00952% Magnesium chloride, 0.00136% Zinc chloride
Affinity purified
Anti-Mouse Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
多克隆
IgG
Abpromise™承诺保证使用ab6790于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | 1/200 - 1/1000. | |
Immunomicroscopy | Use at an assay dependent concentration. | |
WB | 1/1000 - 1/4000. | |
Dot blot | Use at an assay dependent concentration. | |
ELISA | 1/15000. | |
IHC-P | 1/200 - 1/1000. | |
IHC-Fr | Use at an assay dependent concentration. |
Western blot - Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790)
Goat Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6790) at 1/1000 dilution + Mouse IgG
Observed band size: 28,55 kDawhy is the actual band size different from the predicted?
Incubated with ab6790 for 60 min at room temperature.
Blocked for 30 min at room temperature.