Goat Anti-Mouse IgG H&L (TRITC)

• 产品名称

  山羊抗小鼠IgG H&L (TRITC)
  参阅全部 IgG 二抗

• 宿主

  Goat

• 靶标种属

  Mouse

• 经测试应用

  适用于: Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, WB, Dot blot, ELISAmore details

• 免疫原

  Mouse IgG whole molecule

• 偶联物

  TRITC. Ex: 547nm, Em: 572nm

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.01% Sodium azide
  Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride

• 纯度

  Affinity purified

• 纯化说明

  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads.

• 共轭说明

  Tetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab6786于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 

  Flow Cytometry - Goat Anti-Mouse IgG H&L (TRITC) (ab6786)

  Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody Goat anti-mouse IgG H&L (TRITC) (ab6786) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 561nm laser and 610/20 bandpass filter.