山羊抗小鼠IgG H&L (HRP)
参阅全部 IgG 二抗
Goat
Mouse
适用于: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
mouse IgG whole molecule
HRP
Liquid
Shipped at 4°C. Store at +4°C. Store In the Dark.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
1 mg 浓度为 2 mg/ml
Affinity purified
MOUSE IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Horseradish Peroxidase (HRP)
多克隆
IgG
The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
Abpromise™承诺保证使用ab6789于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/500 - 1/2500. | |
| IP | Use at an assay dependent concentration. PubMed: 17164758 | |
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | 1/100000. | |
| IHC-P | 1/500 - 1/2500. | |
| IHC-Fr | 1/500 - 1/2500. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| WB | (3) | 1/2000 - 1/10000. |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/500 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.
All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution
All lanes : Cytosolic fraction prepared from murine brain tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 194 kDawhy is the actual band size different from the predicted?
Exposure time: 1 minute
Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.
ab49969 Immunoprecipitating HA Tag in HEK-293T whole cell lysate. 25 µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)
ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.
