兔抗羊IgG H&L (HRP)
参阅全部 IgG 二抗
Rabbit
Sheep
适用于: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Frmore details
Sheep IgG whole molecule
HRP
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
1 mg 浓度为 2 mg/ml
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
多克隆
IgG
Horseradish Peroxidase (HRP).
Abpromise™承诺保证使用ab6747于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | Use at an assay dependent concentration. | |
WB | (1) | 1/1000 - 1/5000. |
ELISA | Use at an assay dependent concentration. | |
Immunomicroscopy | Use at an assay dependent concentration. | |
Dot blot | Use at an assay dependent concentration. | |
ICC/IF | 1/500 - 1/2500. | |
IHC-Fr | Use at an assay dependent concentration. |
Western blot - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
ab6747 was used at dilution 1/2000 with the primary antibody ab7832 in WB. See the review on ab7832.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)Kälble F et al. Selective Blocking of TNF Receptor 1 Attenuates Peritoneal Dialysis Fluid Induced Inflammation of the Peritoneum in Mice. PLoS One 11:e0163314 (2016).
Representative immunohistochemical staining of parietal peritoneum tissue sections displaying HA in brown color (A-D). HA is dramatically reduced in the peritoneum from mice treated with PDF (B) compared to tissue from untreated mice (A) and mice pretreated with H398 2 hours before application of PDF (C) or PBS (D) (Scale bar: 100μm). The secondary antibody was Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)
Ab6747 was used at dilution 1/100 with the primary antibody ab53842 in IHC-P. See the review on ab53842.