Anti-Bax antibody [5B7]

• 产品名称

  Anti-Bax抗体[5B7]
  参阅全部 Bax 一抗

• 描述

  小鼠单克隆抗体[5B7] to Bax

• 宿主

  Mouse

• 特异性

  Antibody raised against a synthetic peptide that is not shared by rat or human Bax.

• 经测试应用

  适用于: Flow Cyt (Intra), WB, IPmore details

• 种属反应性

  与反应: Mouse
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• WB: A20 and C2C12 lysate. Flow Cyt (intra): RAW 264.7 cells.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 0.5% BSA, PBS, 40% Glycerol (glycerin, glycerine)

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  5B7

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab3191于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 12028 Mouse

• SwissProt: Q07813 Mouse

• Unigene: 19904 Mouse

• 别名

• BAX_HUMAN antibody

• BAXA antibody

• Baxdelta2G9 antibody

• Baxdelta2G9omega antibody

• Baxdelta2omega antibody

• Bcl-2-like protein 4 antibody

• BCL2 associated X protein antibody

• BCL2 associated X protein omega antibody

• BCL2 associated X protein transcript variant delta2 antibody

• Bcl2-L-4 antibody

• BCL2L4 antibody

• membrane isoform alpha antibody

• Apoptosis regulator BAX antibody

• BAX antibody

• Bax-protein antibody

• 

  Western blot - Anti-Bax antibody [5B7] (ab3191)

  Lane 1 : Anti-Bax antibody [5B7] (ab3191) at 0.417 mg/ml
  Lane 2 : Anti-Bax antibody [5B7] (ab3191) at 0.417 µg/ml
  
  Lane 1 : A20 (Mouse reticulum sarcoma B lymphocyte) lysate
  Lane 2 : C2C12 (Mouse myoblasts) lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Predicted band size: 21 kDa
  
  
  Exposure time: 26 seconds
  
  
  

  Blocking/Dilution buffer: 5% NFDM/TBST.

• 

  Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] (ab3191)

  Intracellular flow cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at a 1.043µg/ml  (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

   

• 

  Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)

  Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9µg/ml. Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
  Lane 1: RAW 264.7 whole cell lysate 10ug (Input).
  Lane 2: ab3191 IP in RAW 264.7 whole cell lysate.
  Lane 3: Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
  Blocking/Dilution buffer: 5% NFDM/TBST.
  Exposure time: 30 seconds.

• 

  Anti-Bax antibody [5B7] (ab3191)