Anti-SNX5抗体
参阅全部 SNX5 一抗
山羊多克隆抗体to SNX5
Goat
适用于: IHC-P, WBmore details
与反应: Human
预测可用于: Rat, Cow, Dog, Pig
Synthetic peptide corresponding to Human SNX5 aa 350 to the C-terminus (C terminal). (NP_001269383.1)
Database link: Q9Y5X3
WB: Jurkat cell lysate. IHC-P: Human breast tissue.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab5983于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 3 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | (1) | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). 1 hour primary incubation is recommended for this product. |
Entrez Gene: 27131 Human
Omim: 605937 Human
SwissProt: Q9Y5X3 Human
Unigene: 316890 Human
Unigene: 86151 Rat
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Western blot - Anti-SNX5 antibody (ab5983)
Anti-SNX5 antibody (ab5983) at 1 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 47 kDa
Observed band size: 47 kDa
Primary incubated for 1 hour. Detected using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody (ab5983)
ab5983 at 4µg/ml, staining SNX5 in Human breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with pH 6 citrate buffer was performed, and the sample was stained with HRP.
Western blot - Anti-SNX5 antibody (ab5983)Image courtesy of an anonymous Abreview.
Anti-SNX5 antibody (ab5983) at 1/500 dilution + whole cell lysate prepared from murine macrophages treated with INF gamma at 20 µg
Secondary
HRP conjugated rabbit anti-goat polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 50 kDawhy is the actual band size different from the predicted?
Exposure time: 1 minute
