Anti-Rab9 antibody [Mab9]

• 产品名称

  Anti-Rab9抗体[Mab9]
  参阅全部 Rab9 一抗

• 描述

  小鼠单克隆抗体[Mab9] to Rab9

• 宿主

  Mouse

• 经测试应用

  适用于: WB, IHC-Pmore details
  不适用于: Flow Cyt (Intra)

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Hamster, Cow, Dog, Non human primates

• 免疫原

  Recombinant full length protein corresponding to Dog Rab9.

• 阳性对照

• WB: MDA-MB-231, MCF7, HeLa, HEK293, Jurkat, HepG2 and K562 cell lysates. IHC-P: Human tonsil and spleen tissues.

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: PBS, 6.97% L-Arginine

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• Primary antibody说明

  Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

• 克隆

  单克隆

• 克隆编号

  Mab9

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2810于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for Flow Cyt (Intra).

数据库链接

• Entrez Gene: 511776 Cow

• Entrez Gene: 403947 Dog

• Entrez Gene: 9367 Human

• Entrez Gene: 56382 Mouse

• Entrez Gene: 84589 Rat

• Omim: 300284 Human

• SwissProt: P24408 Dog

• SwissProt: P51151 Human

• SwissProt: Q9R0M6 Mouse

• SwissProt: Q99P75 Rat

• Unigene: 495704 Human

• Unigene: 728400 Human

• Unigene: 25306 Mouse

• Unigene: 35289 Rat

别名

• 2410064E05Rik antibody

• AI195561 antibody

• DmRab9 antibody

• Rab 9 antibody

• RAB 9A antibody

• RAB9 member RAS oncogene family antibody

• RAB9A antibody

• RAB9A member RAS oncogene family antibody

• RAB9A_HUMAN antibody

• RAS ASSOCIATED PROTEIN RAB9 antibody

• Ras related protein Rab 9A antibody

• Ras-related protein Rab-9A antibody

• Sid6061p antibody

• Sid99 antibody

• 

  Western blot - Anti-Rab9 antibody [Mab9] (ab2810)

  All lanes : Anti-Rab9 antibody [Mab9] (ab2810) at 1/1000 dilution
  
  Lane 1 : Wild-type HeLa cell lysate
  Lane 2 : RAB9A knockout HeLa cell lysate
  Lane 3 : MCF7 cell lysate
  Lane 4 : MDA-MB-231 cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
  
  Predicted band size: 22 kDa
  Observed band size: 25 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Lanes 1-4: Merged signal (red and green). Green - ab2810 observed at 25 kDa. Red - loading control ab181602 observed at 36 kDa. 

   ab2810 Anti-Rab9 antibody [Mab9] was shown to specifically react with Rab9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265693 (knockout cell lysate ab257625) was used.  Wild-type and Rab9 knockout samples were subjected to SDS-PAGE.  ab2810 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

• 

  Western blot - Anti-Rab9 antibody [Mab9] (ab2810)

  All lanes : Anti-Rab9 antibody [Mab9] (ab2810) at 10 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 5 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 22 kDa
  Observed band size: 22 kDa
  Additional bands at: 48 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 20 minutes
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)This image is courtesy of an Abreview submitted by Qin Wen

  ab2810 staining Rab9 in Mouse adult testes tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in 1% BSA) for 15 hours at 4°C. A TRITC-conjugated Donkey anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)

  ab2810 (2µg/ml) staining Rab9 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and cell membrane staining.
  Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rab9 antibody [Mab9] (ab2810)

  IHC image of Rab9 staining in human normal tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2810, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX