Anti-Galectin 3 antibody [A3A12]

• 产品名称

  Anti-Galectin 3抗体[A3A12]
  参阅全部 Galectin 3 一抗

• 描述

  小鼠单克隆抗体[A3A12] to Galectin 3

• 宿主

  Mouse

• 特异性

  By Western blot, this antibody detects an ~30 kDa protein representing Galectin 3 from Jurkat cells transfected with human Galectin 3. Immunohistochemical staining of Galectin 3 in rat olfactory bulb yields a pattern consistent with nuclear and plasma membrane staining.

• 经测试应用

  适用于: WB, ICC/IF, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Recombinant full length protein corresponding to Human Galectin 3.

• 表位

  The epitope for this antibody has been mapped to the first 58 amino acids of Galectin 3.

• 阳性对照

• WB: HeLa, MCF7 whole cell lysates. IHC-P: Human ovarian, colon and mouse colon tissue. ICC: HeLa, MCF7 and NIH-3T3 cells.

• 常规说明

   

  This antibody has been shown to potentiate the binding of Galectin 3 to IgG.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: 99% PBS

• 纯度

  Affinity purified

• Primary antibody说明

  This antibody has been shown to potentiate the binding of Galectin 3 to IgG.

• 克隆

  单克隆

• 克隆编号

  A3A12

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2785于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3958 Human

• Entrez Gene: 16854 Mouse

• Omim: 153619 Human

• SwissProt: P17931 Human

• SwissProt: P16110 Mouse

• Unigene: 531081 Human

• Unigene: 248615 Mouse

• 别名

• CBP 35 antibody

• CBP35 antibody

• Gal-3 antibody

• GAL3 antibody

• Galactose-specific lectin 3 antibody

• Galactoside-binding protein antibody

• GALBP antibody

• Galectin 3 internal gene,included antibody

• Galectin-3 antibody

• Galectin3 antibody

• GALIG antibody

• GBP antibody

• IgE binding protein antibody

• IgE-binding protein antibody

• L 31 antibody

• L 34 antibody

• L-31 antibody

• L-34 galactoside-binding lectin antibody

• L31 antibody

• Laminin-binding protein antibody

• Lectin L-29 antibody

• Lectin, galactose binding, soluble 3 antibody

• LEG3_HUMAN antibody

• LGALS2 antibody

• LGALS3 antibody

• MAC 2 antigen antibody

• Mac-2 antibody

• Mac-2 antigen antibody

• MAC2 antibody

• Macrophage galactose-specific lectin antibody

• MGC105387 antibody

• 35 kDa lectin antibody

• Carbohydrate binding protein 35 antibody

• Carbohydrate-binding protein 35 antibody

• 

  Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)

  All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution
  
  Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
  Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
  Lane 4 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
  Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
  Lane 6 : A431 (human epidermoid carcinoma cell line) whole cell lysate
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG (H+L) (HRP) at 1/4000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 26 kDa
  Observed band size: 30 kDawhy is the actual band size different from the predicted?
  Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.
  
  
  
  

  Whole cell extracts were electrophoresed using a 4-12% Bis-Tris Protein Gel. Resolved proteins were then transferred onto a Nitrocellulose membrane using a dry blotting system.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

  Immunohistochemical analysis of human ovarian carcinoma tissue (right) labeling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

  Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Galectin 3 monoclonal antibody (Product # MA1-940) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x

• 

  Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)

  All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution
  
  Lane 1 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
  Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
  
  Lysates/proteins at 25 µg/ml per lane.
  
  Predicted band size: 26 kDa
  Observed band size: 30 kDawhy is the actual band size different from the predicted?
  
  

• 

  Western blot - Anti-Galectin 3 antibody [A3A12] (ab2785)

  All lanes : Anti-Galectin 3 antibody [A3A12] (ab2785) at 1/1000 dilution
  
  Lane 1 : Wild-Type HeLa (human epithelial cell line from cervix adenocarcinoma) membrane extract
  Lane 2 : HeLa CAS9 membrane extract
  Lane 3 : HeLa LGALS3 knockout membrane extract
  
  Lysates/proteins at 30 µg per lane.
  
  Secondary
  All lanes : Goat anti-Mouse IgG (H+L) (HRP) at 1/4000 dilution
  
  Developed using the ECL technique.
  
  Predicted band size: 26 kDa
  
  
  
  

  Knockout of LGALS3 was achieved by CRISPR-Cas9 genome editing using Lentiviral sgRNA and Cas9 Lentivirus. The samples were electrophoresed using 4-12% Bis-Tris Protein Gel. Resolved proteins were then transferred onto a nitrocellulose membrane using a dry blotting system.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

  Immunohistochemical analysis (formalin/PFA-fixed paraffin-embedded sections) human colon tissue (right) labelling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

  Immunofluorescent analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labelling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Galectin 3 antibody [A3A12] (ab2785)

  Immunohistochemical analysis of (formalin/PFA-fixed paraffin-embedded sections) mouse colon tissue (right) labelling Galectin 3 in the nucleus and cytoplasm with ab2785, compared with a negative control (left) by . To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-mouse IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [A3A12] (ab2785)

  Immunofluorescent analysis of MCF7 (Human breast adenocarcinoma cell line) cells labelling Galectin 3 (green) in the cytoplasm and nucleus with ab2785. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.