Anti-IP3 receptor抗体
参阅全部 IP3 receptor 一抗
兔多克隆抗体to IP3 receptor
Rabbit
适用于: ICC/IF, IHC-Pmore details
与反应: Rat, Human
预测可用于: Cow
Synthetic peptide corresponding to Human IP3 receptor aa 1829-1848.
Sequence:
NKKKDDEVDRDAPSRKKAKE
(Peptide available as ab5839)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µl 浓度为 1.3 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5804于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF | Use at an assay dependent concentration. | |
IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Entrez Gene: 3708 Human
Omim: 147265 Human
SwissProt: Q14643 Human
Unigene: 567295 Human
Unigene: 715765 Human
Unigene: 2135 Rat
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Immunocytochemistry/ Immunofluorescence - Anti-IP3 receptor antibody (ab5804)
Shows immunolocalization of ITPR1 in rat cerebellum using ab5804.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody (ab5804)
IHC image of ab5804 staining in normal human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5804, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.