Anti-BACE2 antibody

• 产品名称

  Anti-BACE2抗体
  参阅全部 BACE2 一抗

• 描述

  兔多克隆抗体to BACE2

• 宿主

  Rabbit

• 经测试应用

  适用于: WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide corresponding to Human BACE2 aa 441-457.
  Sequence:

  VSEISGPFSTEDIASNC

  
  (Peptide available as ab5869)

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 99% PBS, 0.1% BSA

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab5670于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 25825 Human

• Entrez Gene: 56175 Mouse

• Omim: 605668 Human

• SwissProt: Q9Y5Z0 Human

• SwissProt: Q9JL18 Mouse

• Unigene: 529408 Human

• Unigene: 97885 Mouse

• 别名

• ASP1 antibody

• ASP21 antibody

• Aspartic Like Protease 56kDa antibody

• Aspartic-like protease 56 kDa antibody

• Aspartyl protease 1 antibody

• BACE 2 antibody

• Bace2 antibody

• BACE2_HUMAN antibody

• Beta Secretase 2 antibody

• Beta Site Amyloid Beta A4 Precursor Protein Cleaving Enzyme 2 antibody

• Beta-secretase 2 antibody

• Beta-site amyloid precursor protein cleaving enzyme 2 antibody

• Beta-site APP cleaving enzyme 2 antibody

• Down region aspartic protease antibody

• Downs Syndrome Region Aspartic Protease antibody

• DRAP antibody

• Memapsin 1 antibody

• Memapsin-1 antibody

• Membrane associated aspartic protease 1 antibody

• Membrane-associated aspartic protease 1 antibody

• Theta-secretase antibody

• ALP 56 antibody

• ALP56 antibody

• Asp 1 antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-BACE2 antibody (ab5670)

  Immunocytochemistry/Immunofluorescence analysis of BACE2 (green) showing staining in the nucleus and cytoplasm of A549 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab5670 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

• 

  Western blot - Anti-BACE2 antibody (ab5670)

  All lanes : Anti-BACE2 antibody (ab5670) at 1/500 dilution
  
  Lane 1 : Mouse spleen cell lysate
  Lane 2 : SH-SY5Y cell lysate
  Lane 3 : HL60 cell lysate
  Lane 4 : HeLa cell lysate
  
  Lysates/proteins at 25 µg per lane.