Anti-PKC gamma (phospho T655)抗体
参阅全部 PKC gamma 一抗
兔多克隆抗体to PKC gamma (phospho T655)
Rabbit
PKC alpha (69%) and beta 2 (62%) may cross-react in cells expressing high levels of these proteins.
适用于: IHC-P, WBmore details
与反应: Mouse, Human
预测可用于: Rat
Synthetic peptide corresponding to PKC gamma (phospho T655).
WB: HeLa cells treated with PMA, a phorbol ester. IHC-P: Mouse cerebellum tissue, human cerebellum tissue.
PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Immunogen affinity purified
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC gamma. The final product is generated by affinity chromatography using a PKC gamma-derived peptide that is phosphorylated at threonine 655.
PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma.
多克隆
IgG
Abpromise™承诺保证使用ab5796于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/10 - 1/50. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 80 kDa. |
Entrez Gene: 5582 Human
Entrez Gene: 18752 Mouse
Omim: 176980 Human
SwissProt: P05129 Human
SwissProt: P63318 Mouse
Unigene: 631564 Human
Unigene: 7980 Mouse
Unigene: 9747 Rat
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Western blot - Anti-PKC gamma (phospho T655) antibody (ab5796)
Peptide Competition and Phosphatase treatment: Lysates prepared from HeLa cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-11) or treated with lambda (ë) phosphatase (12), blocked with a 3% low-fat milk-TBST buffer overnight at 4°C, and incubated with 0.50 µg/mL ab5796 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 11, 12), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), the phosphopeptide immunogen (4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (5-10). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStarTM method. The data show that the peptide corresponding to PKC gamma [pT655] blocks the an
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)
Paraffin embedded human cerebellum tissue (right) stained for PKC gamma using ab5796 at 1/20 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)
Paraffin embedded mouse cerebellum tissue (right) stained for PKC gamma using ab5796 at 1/100 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
