Anti-KIF5A抗体
参阅全部 KIF5A 一抗
兔多克隆抗体to KIF5A
Rabbit
This antibody is specific for KIF5A and does not detect other kinesin isotypes.
适用于: ICC/IF, WB, IPmore details
与反应: Mouse, Rat, Cow, Human
Synthetic peptide corresponding to Mouse KIF5A aa 1007-1027.
Sequence:
CGYEAEDQAKLFPLHQETAAS
(Peptide available as ab41781)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
多克隆
IgG
Abpromise™承诺保证使用ab5628于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| WB | (2) | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 117 kDa. |
| IP | (1) | Use at an assay dependent concentration. PubMed: 20094756 |
Entrez Gene: 3798 Human
Entrez Gene: 16572 Mouse
Omim: 602821 Human
SwissProt: Q12840 Human
SwissProt: P33175 Mouse
Unigene: 151219 Human
Unigene: 30355 Mouse
Unigene: 63144 Rat
D12S1889 antibody
KIF 5A antibody
Kif5a antibody
KIF5A_HUMAN antibody
Kinesin family member 5A antibody
Kinesin heavy chain isoform 5A antibody
Kinesin Heavy Chain Neuron Specific antibody
Kinesin heavy chain neuron-specific 1 antibody
MY050 antibody
Neuronal kinesin heavy chain antibody
NKHC 1 antibody
NKHC antibody
SPG 10 antibody
Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)
Immunofluorescence analysis of KIF5A in SH-SY5Y cells using ab5628. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.25% Triton™ X-100, and blocked with 5% BSA. The cells were labeled with ab5628 at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. (Panel d) represents the merged image showing cytoplasmic localization. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.
Western blot - Anti-KIF5A antibody (ab5628)
All lanes : Anti-KIF5A antibody (ab5628) at 2 µg/ml
Lane 1 : U-87 MG whole cell extracts
Lane 2 : HT-29 whole cell extracts
Lane 3 : C2C12 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Predicted band size: 117 kDa
Blocking with 5 % skimmed milk
Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)
ICC/IF image of ab5628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5628, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
