Anti-KIF5C抗体
参阅全部 KIF5C 一抗
兔多克隆抗体to KIF5C
Rabbit
This antibody is specific for KIF5C and does not detect other kinesin isotypes.
适用于: IHC-P, WBmore details
与反应: Human
Synthetic peptide corresponding to Human KIF5C aa 938-957.
Sequence:
AVHAIRGGGGSSSNSTHYQK
(Peptide available as ab41784)
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
多克隆
IgG
Abpromise™承诺保证使用ab5630于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | (4) | Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
Entrez Gene: 3800 Human
Omim: 604593 Human
SwissProt: O60282 Human
Unigene: 435557 Human
Unigene: 660699 Human
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Western blot - Anti-KIF5C antibody (ab5630)
Shows a Western blot of KIF5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody (ab5630)
IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
