兔抗亚美尼亚仓鼠IgG H&L (HRP)
参阅全部 IgG 二抗
Rabbit
Armenian hamster
Greatly diminished reactivity will occur against Golden Syrian Hamster IgG.
适用于: Dot blot, WB, ELISA, Electron Microscopy, IHC-P, ICCmore details
Armenian Hamster IgG whole molecule.
HRP
Liquid
Shipped at 4°C. Store at +4°C.
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
BSA is IgG and protease free
浓度
1 mg 浓度为 1 mg/ml
Affinity purified
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose.
Horseradish Peroxidase (HRP).
多克隆
IgG
Abpromise™承诺保证使用ab5745于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Dot blot | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/5000. | |
| ELISA | 1/150000 - 1/250000. This product has been assayed against 1.0 ug of Armenian Hamster IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. | |
| Electron Microscopy | Use at an assay dependent concentration. | |
| IHC-P | (1) | 1/1000 - 1/5000. |
| ICC | 1/1000 - 1/5000. |
ELISA - Rabbit Anti-Armenian hamster IgG H&L (HRP) (ab5745)
ab5745 tested against purified Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of Armenian Hamster IgG. The starting dilution of antibody was 5µg/ml and the X-axis represents the log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate
