Anti-PAX6抗体
参阅全部 PAX6 一抗
兔多克隆抗体to PAX6
Rabbit
This antibody does not detect bovine PAX6 by Western blot.
We have had mixed results for use of this antibody in IHC-Fr. Thus, we are removing IHC-Fr as a guaranteed application and welcome any feedback from customers who have used this antibody in IHC-Fr.
适用于: IP, ICC/IF, WBmore details
与反应: Mouse, Rat, Human, Monkey
预测可用于: Chicken, Xenopus laevis, Non human primates, Zebrafish
Synthetic peptide corresponding to Mouse PAX6 aa 267-285.
Sequence:
REEKLRNQRRQASNTPSHI
ICC: ATRA treated NCCIT cells; WB: rat whole eye extract; human 293T, HepG2, U20S, MCF7, Jurkat NCCIT cells; monkey COS7, mouse C2C12 cells; IP: 293T cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Immunogen affinity purified
PAX genes encode nuclear transcription factors which are regarded as major controllers of developmental processes in both vertebrates and invertebrates. Mutations in murine PAX genes underlie three natural mouse alleles and several corresponding human syndromes (aniridia, foveal hypoplasia and Peters’ anomaly). Murine PAX genes have been shown to be proto-oncogenes. Furthermore, human PAX genes have recently been demonstrated to play an influential part in some common human cancers such as brain tumors and lymphomas. All PAX genes encode a DNA-binding domain termed the paired domain and in addition some also encode a second binding domain--the paired type homeobox. PAX6 is involved in the early development of the optical vesicle and has been shown to interact with Six3, another important visual development protein.
多克隆
IgG
Abpromise™承诺保证使用ab5790于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use a concentration of 5 µg/ml. | |
| ICC/IF | (7) | 1/50. |
| WB | (6) | 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 50.6 kDa). This antibody can be blocked with ab5895. |
Entrez Gene: 5080 Human
Entrez Gene: 18508 Mouse
Entrez Gene: 30567 Zebrafish
Omim: 607108 Human
SwissProt: P26367 Human
SwissProt: P63015 Mouse
SwissProt: P55864 Xenopus laevis
SwissProt: P26630 Zebrafish
Unigene: 270303 Human
Unigene: 611376 Human
Unigene: 33870 Mouse
Unigene: 3608 Mouse
Unigene: 89724 Rat
Unigene: 647 Xenopus laevis
Unigene: 24244 Zebrafish
AN 2 antibody
AN antibody
AN2 antibody
Aniridia type II protein antibody
D11S812E antibody
FVH1 antibody
KIAA0552 antibody
Leucine zipper putative tumor suppressor 3 antibody
LZTS3 antibody
MGC17209 antibody
MGDA antibody
Oculorhombin antibody
Paired box 6 antibody
Paired box gene 6 (aniridia keratitis) antibody
Paired Box Gene 6 antibody
Paired box homeotic gene 6 antibody
Paired box protein Pax-6 antibody
Paired box protein Pax6 antibody
PAX 6 antibody
PAX6 antibody
PAX6_HUMAN antibody
ProSAP-interacting protein 1 antibody
PROSAPIP1 antibody
Sey antibody
WAGR antibody
Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)
Immunofluorescent analysis of PAX6 (green) in All-Trans-Retinoic Acid (ATRA) treated (10 µM for 24 hours) NCCIT cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody(ab5790) at a dilution of 1:50 for at least 1 hour at room temperature and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Western blot - Anti-PAX6 antibody (ab5790)
Western blot analysis of PAX6 was performed by loading 50ug of various Human, mouse and non-human primate whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a PAX6 polyclonal antibody (ab5790) at a dilution of 1:1000 overnight at 4°C on a rocking platform then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:20000 for at least one hour. Chemiluminescent detection was performed.
Immunoprecipitation - Anti-PAX6 antibody (ab5790)
Immunoprecipitation of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500 µg whole cell lysate with 5 µg of PAX6 polyclonal antibody (ab5790) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 µl Protein A/G Agarose, washed extensively and eluted with 5X Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel then transferred to a PVDF membraneand blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4°C then washed in TBST and probed with detection reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed.
