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Anti-Blooms Syndrome Protein Blm antibody

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50uL
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  • 产品名称

    Anti-Blooms Syndrome蛋白Blm抗体
    参阅全部 Blooms Syndrome Protein Blm 一抗

  • 描述

    山羊多克隆抗体to Blooms Syndrome蛋白Blm

  • 宿主

    Goat

  • 经测试应用

    适用于: WBIPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Chimpanzee

  • 免疫原

    Immunogen was a synthetic peptide, which represented a portion of human Bloom Syndrome encoded within exon 2.

  • 阳性对照

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.

  • 常规说明


    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • 存储溶液

    pH: 7
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

  • 浓度

    • 50 µl 浓度为 1 mg/ml

  • 纯度

    Immunogen affinity purified

  • 克隆

    多克隆

  • 同种型

    IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab5446于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用Ab评论说明
WB(2)

1/10000 - 1/25000. Predicted molecular weight: 159 kDa.

IP

Use at 2-10 µg/mg of lysate.

  • 数据库链接

  • 别名

    • Bloom syndrome protein antibody

    • Bloom syndrome RecQ helicase like antibody

    • BS antibody

    • DNA helicase antibody

    • DNA helicase RecQ like type 2 antibody

    • MGC126616 antibody

    • MGC131618 antibody

    • MGC131620 antibody

    • RECQ 2 antibody

    • RECQ like antibody

    • RecQ like type 2 antibody

    • RecQ protein like 3 antibody

    • RecQ protein-like 3 antibody

    • RecQ-like type 2 antibody

    • RecQ2 antibody

    • RECQL 2 antibody

    • RECQL 3 antibody

    • RECQL2 antibody

    • RECQL3 antibody

    • type 2 antibody

    • Blm antibody

    • BLM_HUMAN antibody

    • Bloom syndrome antibody

  • Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)

    Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)

    All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab5446) at 0.04 µg/ml

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 159 kDa


    Exposure time: 30 seconds

  • Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)

    Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)

    Blooms Syndrome Protein Blm was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab5446 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab5446 at 1 µg/ml. 

    Lane 1: ab5446 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.