Anti-Blooms Syndrome蛋白Blm抗体
参阅全部 Blooms Syndrome Protein Blm 一抗
山羊多克隆抗体to Blooms Syndrome蛋白Blm
Goat
适用于: WB, IPmore details
与反应: Human
预测可用于: Chimpanzee
Immunogen was a synthetic peptide, which represented a portion of human Bloom Syndrome encoded within exon 2.
WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µl 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab5446于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) | 1/10000 - 1/25000. Predicted molecular weight: 159 kDa. |
IP | Use at 2-10 µg/mg of lysate. |
Entrez Gene: 641 Human
Omim: 604610 Human
SwissProt: P54132 Human
Unigene: 725208 Human
Bloom syndrome protein antibody
Bloom syndrome RecQ helicase like antibody
BS antibody
DNA helicase antibody
DNA helicase RecQ like type 2 antibody
MGC126616 antibody
MGC131618 antibody
MGC131620 antibody
RECQ 2 antibody
RECQ like antibody
RecQ like type 2 antibody
RecQ protein like 3 antibody
RecQ protein-like 3 antibody
RecQ-like type 2 antibody
RecQ2 antibody
RECQL 2 antibody
RECQL 3 antibody
RECQL2 antibody
RECQL3 antibody
type 2 antibody
Blm antibody
BLM_HUMAN antibody
Bloom syndrome antibody
Western blot - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
All lanes : Anti-Blooms Syndrome Protein Blm antibody (ab5446) at 0.04 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 159 kDa
Exposure time: 30 seconds
Immunoprecipitation - Anti-Blooms Syndrome Protein Blm antibody (ab5446)
Blooms Syndrome Protein Blm was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab5446 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab5446 at 1 µg/ml.
Lane 1: ab5446 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.