Anti-PDGFR alpha (phospho Y742)抗体
参阅全部 PDGFR alpha 一抗
兔多克隆抗体to PDGFR alpha (phospho Y742)
Rabbit
PDGFR beta (50% homologous) has not been tested, but is not expected to react.
适用于: WBmore details
与反应: Mouse
预测可用于: Rat, Human, Xenopus laevis
Synthetic peptide corresponding to Human PDGFR alpha (phospho Y742).
NIH3T3 cells +/- PDGF.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free
Immunogen affinity purified
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at Tyrosine 742.
多克隆
IgG
Abpromise™承诺保证使用ab5452于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 170-185 kDa. |
Entrez Gene: 5156 Human
Entrez Gene: 18595 Mouse
Omim: 173490 Human
SwissProt: P16234 Human
SwissProt: P26618 Mouse
SwissProt: P26619 Xenopus laevis
Unigene: 74615 Human
Unigene: 221403 Mouse
Unigene: 55127 Rat
Unigene: 20029 Xenopus laevis
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Western blot - Anti-PDGFR alpha (phospho Y742) antibody (ab5452)
Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5452 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the nonphosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5452 blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5)
