Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [8A7]

• 产品名称

  Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5)抗体[8A7]
  参阅全部 RNA polymerase II CTD repeat YSPTSPS 一抗

• 描述

  小鼠单克隆抗体[8A7] to RNA polymerase II CTD repeat YSPTSPS (phospho S5)

• 宿主

  Mouse

• 经测试应用

  适用于: Flow Cytmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse

• 免疫原

  Synthetic peptide corresponding to Human RNA polymerase II CTD repeat YSPTSPS (phospho S5). Synthetic peptide: 10 repeats of YSPTSpPS (Human).
  Sequence: YSPTS_pPS
  Database link: P24928

  Run BLAST with Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

• 存储溶液

  Preservative: 0.02% Sodium azide
  Constituent: 99.98% PBS

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  8A7

• 骨髓瘤

  Sp2/0-Ag14

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab5401于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 5430 Human

• Entrez Gene: 20020 Mouse

• Omim: 180660 Human

• SwissProt: P24928 Human

• SwissProt: P08775 Mouse

• Unigene: 270017 Human

• Unigene: 16533 Mouse

• 别名

• DNA-directed RNA polymerase II subunit RPB1 antibody

• DNA-directed RNA polymerase III largest subunit antibody

• hRPB220 antibody

• hsRPB1 antibody

• POLR2 antibody

• Polr2a antibody

• POLRA antibody

• Polymerase (RNA) II (DNA directed) polypeptide A 220kDa antibody

• Polymerase (RNA) II (DNA directed) polypeptide A antibody

• RNA polymerase II subunit B1 antibody

• RNA-directed RNA polymerase II subunit RPB1 antibody

• RPB1 antibody

• RPB1_HUMAN antibody

• RPBh1 antibody

• RpIILS antibody

• RPO2 antibody

• RPOL2 antibody

• DNA directed RNA polymerase II A antibody

• DNA-directed RNA polymerase II largest subunit RNA polymerase II 220 kd subunit antibody

• DNA-directed RNA polymerase II subunit A antibody

Flow Cytometry - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody [8A7] (ab5401)

Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab5401 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5401, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.