HRP Anti-Interferon alpha 2b antibody [4E10]

• 产品名称

  HRP Anti-Interferon alpha 2b抗体[4E10]
  参阅全部 Interferon alpha 2b 一抗

• 描述

  HRP小鼠单克隆抗体[4E10] to Interferon alpha 2b

• 宿主

  Mouse

• 偶联物

  HRP

• 特异性

  Recognizes both human recombinant IFN-a2b and IFN-a2a. Does not react with human recombinant IFN-a5 and IFN-g.

• 经测试应用

  适用于: ELISAmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human Interferon alpha 2b.

• 常规说明

  Recommended ELISA procedure for human IFN alpha 2b determination: 1. Use 96-well Nunc Maxisorp plates for the assay. Coat microwells with 100 µl/well of antibody 9D3 (ab9386) diluted to 10 µg/ml in coating buffer (0.05 M Na-carbonate, pH 9.5). Incubate overnight at +4°C. 2. Aspirate wells and block with 150 µl/well of blocking buffer (2% BSA in PBS). Incubate at RT for 1 h. 3. Aspirate wells and wash 3 times with PBST (0.1% Tween-20 in PBS). 4. Prepare standard and sample dilutions in PBST. Recommended IFN alpha 2b concentrations: from 100 pg/ml to 100 ng/ml. Add 100 µl of each standard and sample into appropriate wells. Incubate at RT for 2 h. 5. Aspirate and wash 5 times with PBST. 6. Dilute detection antibody 4E10-HRP (ab5258) 1:1000 in PBST. Add 100 µl of diluted detection antibody to each well. Incubate at RT for 1 h. 7. Aspirate and wash 10 times with PBST. 8. Add 100 µl of substrate solution (TMB) to each well. Incubate at RT for 10-20 min. 9. Add 50 µl of stop solution (2 N H2SO4) to each well. Read absorbance at 450 nm.

  This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR308991-1 and GR308991-2 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

• 浓度

• 100 µl 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  4E10

• 骨髓瘤

  NS0/1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab5258于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 3440 Human

• Omim: 147562 Human

• SwissProt: P01563 Human

• Unigene: 211575 Human

• 别名

• IFNA antibody

• Ifna2 antibody

• IFNA2_HUMAN antibody

• INFA2 antibody

• Interferon alpha 2 antibody

• Interferon alpha A antibody

• Interferon alpha-2 antibody

• Interferon alpha-A antibody

• LeIF A antibody

• LeIFA antibody

• Alpha 2a interferon antibody

• IFN alpha 2b antibody

• IFN-alpha-2 antibody