Anti-IRS1 (phospho S312)抗体
参阅全部 IRS1 一抗
兔多克隆抗体to IRS1 (phospho S312)
Rabbit
适用于: WBmore details
与反应: Human
预测可用于: Mouse, Rat, Pig
Synthetic peptide corresponding to Human IRS1 (phospho S312).
Phorbol ester stimulated (TPA) Chinese Hamster Ovary cell line expressing human insulin receptor (CHO-T) and transiently transfected with a plasmid encoding human IRS 1.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, Glycerol (glycerin, glycerine), 0.1% BSA
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated IRS 1. The final product is generated by affinity chromatography using an IRS 1-derived peptide phosphorylated at serine 312.
多克隆
IgG
Abpromise™承诺保证使用ab4865于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 165 kDa. |
Entrez Gene: 3667 Human
Entrez Gene: 16367 Mouse
Omim: 147545 Human
SwissProt: P35568 Human
SwissProt: P35569 Mouse
Unigene: 471508 Human
Unigene: 4952 Mouse
Unigene: 10476 Rat
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Western blot - Anti-IRS1 (phospho S312) antibody (ab4865)
Peptide Competition and Phosphatase Stripping: Extracts prepared from CHO-T cells transiently transfected with wild-type human IRS 1 and treated with TPA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were treated with lambda phosphatase (1) or left untreated (2-6), blocked with a 5% BSA-TBST buffer overnight at 4oC, then incubated with 0.50 µg/mL ab4865 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1-3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab4865 blocks the antibody signal, thereby demonstrating the specificity of the anti
