Anti-PAK1 + PAK2 + PAK3 (phospho S141)抗体
参阅全部 PAK1 + PAK2 + PAK3 一抗
兔多克隆抗体to PAK1 + PAK2 + PAK3 (phospho S141)
Rabbit
适用于: WBmore details
与反应: Human
预测可用于: Rat, Rabbit
Synthetic peptide corresponding to Human PAK1 + PAK2 + PAK3 (phospho S141).
SH-SY5Y, SH-SY5Y treated with 100 uM Pervanadate, HeLa, HeLa treated with 100 uM Pervanadate, K562, K562 treated with 100 uM Pervanadate
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PAK. The final product is generated by affinity chromatography using a PAK 2 derived peptide that is phosphorylated at serine 141.
P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.
多克隆
IgG
Abpromise™承诺保证使用ab5247于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 65 kDa. |
Entrez Gene: 5058 Human
Entrez Gene: 5062 Human
Entrez Gene: 5063 Human
Omim: 300142 Human
Omim: 602590 Human
Omim: 605022 Human
SwissProt: O75914 Human
SwissProt: Q13153 Human
SwissProt: Q13177 Human
SwissProt: Q29502 Rabbit
Alpha PAK antibody
Beta PAK antibody
bPAK antibody
CDKN1A antibody
Gamma PAK antibody
hPAK3 antibody
MRX30 antibody
MRX47 antibody
Oligophrenin 3 antibody
OPHN3 antibody
P21 (CDKN1A) activated kinase 2 antibody
P21 (CDKN1A) activated kinase 3 antibody
p21 activated kinase 1 antibody
p21 activated kinase 2 antibody
p21 activated kinase 3 antibody
P21 protein (Cdc42/Rac) activated kinase 1 antibody
P21 protein (Cdc42/Rac) activated kinase 2 antibody
P21 protein (Cdc42/Rac) activated kinase 3 antibody
P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog, yeast) antibody
P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
P58 antibody
P65 PAK antibody
p65-PAK antibody
PAK 2 antibody
PAK 3 antibody
PAK1 antibody
PAK2 antibody
PAK3 antibody
PAK3beta antibody
PAK65 antibody
PAKalpha antibody
PAKgamma antibody
S6/H4 kinase antibody
Serine/threonine protein kinase PAK 1 antibody
Serine/threonine protein kinase PAK 2 antibody
Serine/threonine protein kinase PAK 3 antibody
Serine/threonine protein kinase PAK1 antibody
Serine/threonine protein kinase PAK2 antibody
Serine/threonine protein kinase PAK3 antibody
Serine/threonine-protein kinase PAK 1 antibody
STE20 homolog, yeast antibody
Western blot - Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247)
All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho S141) antibody (ab5247) at 1/500 dilution
Lane 1 : SH-SY5Y whole cell extracts
Lane 2 : SH-SY5Y treated with 100 uM Pervanadate for 1 h
Lane 3 : HeLa whole cell extract
Lane 4 : HeLa treated with 100 uM Pervanadate for 1 h
Lane 5 : K562 whole cell extract
Lane 6 : K562 treated with 100 uM Pervanadate for 1 h
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution
Predicted band size: 65 kDa
