Anti-eNOS antibody

• 产品名称

  Anti-eNOS抗体
  参阅全部 eNOS 一抗

• 描述

  兔多克隆抗体to eNOS

• 宿主

  Rabbit

• 特异性

  This antibody does not detect human inducible NOS or rat brain nNOS. ab5589 has been successfully used in Western blot and immunohistochemistry (paraffin and frozen) procedures. By Western blot, this antibody detects an ~140 kDa protein representing eNOS from human endothelial cells. Immunohistochemical staining of eNOS in Human vascular endothelial cells with ab5589 results in prominent staining of the epithelial cells.

• 经测试应用

  适用于: IHC-Pmore details
  不适用于: WB

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Synthetic peptide corresponding to Human eNOS aa 1179-1194.
  Sequence:

  SLQERQLRGAVPWAFD

  Run BLAST with Run BLAST with 

• 阳性对照

• IHC-P: Human lung, placenta and spleen tissues.

• 常规说明

  We have received mixed feedback from customers regarding rat specificity; this is why we are temporarily removing Rat from tested species list and no longer guarantee it. Our laboratory is currently testing new lots so please contact our scientific support team (technical@abcam.com) for more information.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µl 浓度为 0.2 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab5589于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for WB.

数据库链接

• Entrez Gene: 4846 Human

• Entrez Gene: 18127 Mouse

• Entrez Gene: 24600 Rat

• Omim: 163729 Human

• SwissProt: P29474 Human

• SwissProt: P70313 Mouse

• SwissProt: Q62600 Rat

• Unigene: 647092 Human

• Unigene: 258415 Mouse

• Unigene: 44265 Rat

别名

• cNOS antibody

• Constitutive NOS antibody

• EC NOS antibody

• EC-NOS antibody

• ecNOS antibody

• Endothelial nitric oxidase synthase antibody

• Endothelial nitric oxide synthase antibody

• Endothelial nitric oxide synthase 3 antibody

• Endothelial NOS antibody

• eNOS antibody

• Nitric oxide synthase 3 (endothelial cell) antibody

• Nitric oxide synthase 3 antibody

• Nitric oxide synthase 3 endothelial cell antibody

• Nitric oxide synthase endothelial antibody

• Nitric oxide synthase, endothelial antibody

• NOS 3 antibody

• NOS III antibody

• NOS type III antibody

• NOS3 antibody

• NOS3_HUMAN antibody

• NOSIII antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)

  Immunolocalization of eNOS in human lung tissue using ab5589.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)

  IHC image of eNOS staining in human placenta formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5589, 1/100, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

   

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-eNOS antibody (ab5589)

  The specificity of ab5589 was demonstrated using naturally occurring variable expression to confirm specificity. Immunohistochemistry analysis of human tissue sections using this antibody showed eNOS is strongly stained in human spleen tissue and no staining in human tonsil tissue which does not express eNOS.