Anti-Mast Cell Chymase antibody [CC1]

• 产品名称

  Anti-Mast Cell Chymase抗体[CC1]
  参阅全部 Mast Cell Chymase 一抗

• 描述

  小鼠单克隆抗体[CC1] to Mast Cell Chymase

• 宿主

  Mouse

• 经测试应用

  适用于: IHC-Pmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 1st November 2024.

  Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.2
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

• 纯度

  Protein A purified

• Primary antibody说明

  Mast cells contain a number of preformed chemicals mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. Human Mast Cell Chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.

• 克隆

  单克隆

• 克隆编号

  CC1

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab2377于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1215 Human

• Omim: 118938 Human

• SwissProt: P23946 Human

• Unigene: 135626 Human

• 别名

• chymase 1 preproprotein transcript E antibody

• chymase 1 preproprotein transcript I antibody

• Chymase antibody

• Chymase, heart antibody

• Chymase, mast cell antibody

• CMA1 antibody

• CMA1_HUMAN antibody

• CYH antibody

• CYM antibody

• EC 3.4.21.39 antibody

• Mast cell chymase 1 antibody

• Mast cell protease 3 antibody

• Mast cell protease 5 antibody

• Mast cell protease I antibody

• Mast cell protease III antibody

• Mcp-5 antibody

• MCP3P antibody

• Mcpt5 antibody

• MCT1 antibody

• MGC119890 antibody

• MGC119891 antibody

• MMCP-5 antibody

• Alpha-chymase antibody

• Chymase 1 antibody

• Chymase 1 mast cell antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mast Cell Chymase antibody [CC1] (ab2377)

  IHC image of Mast Cell Chymase staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2377, 0.2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

   

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre