Anti-TopBP1抗体
参阅全部 TopBP1 一抗
兔多克隆抗体to TopBP1
Rabbit
适用于: WB, IPmore details
与反应: Mouse, Human
Synthetic peptide within Human TopBP1 aa 1400-1500. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_008958.1 (GeneID 11073).
Database link: Q92547
WB: HeLa, HEK-293T, TCMK-1, NIH/3T3 and Jurkat whole cell lysate. IP: TopBP1 in HeLa whole cell lysate.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Immunogen affinity purified
Antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
多克隆
IgG
Abpromise™承诺保证使用ab2402于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (8) | 1/2000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 173.3 kDa). |
| IP | (2) | Use at 2-5 µg/mg of lysate. |
Entrez Gene: 11073 Human
Entrez Gene: 235559 Mouse
Omim: 607760 Human
SwissProt: Q92547 Human
SwissProt: Q6ZQF0 Mouse
Unigene: 593379 Human
Unigene: 259893 Mouse
DNA topoisomerase II binding protein antibody
DNA topoisomerase II-beta-binding protein 1 antibody
DNA topoisomerase II-binding protein 1 antibody
DNA topoisomerase IIbeta binding protein 1 antibody
Hypothetical protein KIAA0259 [Fragment] antibody
KIAA0259 antibody
TOP2BP1 antibody
TOPB1_HUMAN antibody
TOPBP 1 antibody
TopBP1 antibody
Topoisomerase (DNA) II binding protein 1 antibody
Topoisomerase II binding protein 1 antibody
DNA topoisomerase 2 binding protein 1 antibody
DNA topoisomerase 2-binding protein 1 antibody
DNA topoisomerase II binding protein 1 antibody
Western blot - Anti-TopBP1 antibody (ab2402)
All lanes : Anti-Cytokeratin 10 antibody [LHP1] (ab2409) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 173.3 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-TopBP1 antibody (ab2402)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Antibodies: ab2409 used for IP at 3 µg per reaction.
Two lots tested (Lanes 1 and 2).
Lane 3 is Control IgG.
For blotting immunoprecipitated TopBP1, ab2409 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western blot - Anti-TopBP1 antibody (ab2402)
All lanes : Anti-Cytokeratin 10 antibody [LHP1] (ab2409) at 0.1 µg/ml
Lane 1 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 173.3 kDa
Exposure time: 3 minutes
Lysates prepared using NETN lysis buffer.
