Anti-KAP1抗体
参阅全部 KAP1 一抗
山羊多克隆抗体to KAP1
Goat
适用于: ICC/IF, IHC-P, WBmore details
与反应: Mouse, Human
Synthetic peptide: SSQELSGGPGDGP, corresponding to amino acids 741-753 of KAP 1.
WB: HepG2 lysate. IHC-P: Human breast tissue.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab3831于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | Use a concentration of 5 µg/ml. |
| IHC-P | Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | (2) | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa). 1 hour primary incubation is recommended for this product. |
Entrez Gene: 10155 Human
Entrez Gene: 21849 Mouse
Omim: 601742 Human
SwissProt: Q13263 Human
SwissProt: Q62318 Mouse
Unigene: 467408 Human
Unigene: 15701 Mouse
Unigene: 398345 Mouse
KAP 1 antibody
KAP-1 antibody
KRAB associated protein 1 antibody
KRAB interacting protein 1 antibody
KRAB-associated protein 1 antibody
KRAB-interacting protein 1 antibody
KRIP 1 antibody
KRIP-1 antibody
KRIP1 antibody
Nuclear corepressor KAP 1 antibody
Nuclear corepressor KAP-1 antibody
RING finger protein 96 antibody
RNF96 antibody
TF1B antibody
TIF1 beta antibody
TIF1-beta antibody
TIF1B antibody
TIF1B_HUMAN antibody
Transcription intermediary factor 1 beta antibody
Transcription intermediary factor 1-beta antibody
Trim28 antibody
Tripartite motif containing 28 antibody
tripartite motif containing protein 28 antibody
Tripartite motif-containing protein 28 antibody
E3 SUMO protein ligase TRIM28 antibody
E3 SUMO-protein ligase TRIM28 antibody
FLJ29029 antibody
Western blot - Anti-KAP1 antibody (ab3831)
ab3831 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab3831 staining (0.5µg/ml) of HepG2 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunocytochemistry/ Immunofluorescence - Anti-KAP1 antibody (ab3831)
TIF1b is uniformly distributed in the two pronuclei of the mouse embryo at the late zygote stage. Mouse zygotes were processed for immunostaining using the KAP1 antibody. DNA is shown in blue. The dilution used was 1 to 100 in PBS-Tween with 3% BSA. This is part of the review submitted by ME Torres-Padilla on 27 July 2004
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 antibody (ab3831)
Paraffin embedded human breast tissue stained for KAP1 using ab3831 at 2 µg/ml in immunohistochemical analysis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
