Anti-PYK2 (phospho Y881)抗体
参阅全部 PYK2 一抗
兔多克隆抗体to PYK2 (phospho Y881)
Rabbit
Some cross-reactivity with Focal Adhesion Kinase phosphorylated at tyrosine 925 (the corresponding Grb 2 binding site) may be observed in cases where Focal Adhesion Kinase is overexpressed relative to PYK 2 in the same cell system.
适用于: WBmore details
与反应: Chicken
预测可用于: Mouse, Rat
Synthetic peptide corresponding to Human PYK2 (phospho Y881).
WB: Chicken embryo fibroblasts plated on fibronectin
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Immunogen affinity purified
Purified from rabbit serum by epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PYK 2. The final product is generated by affinity chromatography using a PYK 2-derived peptide that is phosphorylated at tyrosine 881.
多克隆
IgG
Abpromise™承诺保证使用ab4801于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
应用说明
IHC-Fr: Use at an assay dependent dilution.
WB: 1/1000. Predicted molecular weight: 116 kDa. Can be blocked with PYK 2 (phospho) antibody panel or CEF whole cell lysate, positive / negative for PYK 2 (pair).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Entrez Gene: 421998 Chicken
Entrez Gene: 19229 Mouse
SwissProt: Q9QVP9 Mouse
Unigene: 21613 Mouse
Unigene: 11025 Rat
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Protein kinase B antibody
Protein Tyrosine Kinase 2 Beta antibody
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Western blot - Anti-PYK2 (phospho Y881) antibody (ab4801)
All lanes : Anti-PYK2 (phospho Y881) antibody (ab4801) at 1/1000 dilution
Lane 1 : Mock-transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 2 : Only expression vector transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 3 : Pyk2 (pY^881)NP expression vector transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 4 : FAK (pY^925) expression vector transfected- Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 5 : Generic pY expression vector transfected - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Lane 6 : Pyk2 (pY^881)P - Primary chicken embryo fibroblasts plated on fibronectin, pervanadate-treated
Predicted band size: 116 kDa
