Anti-FAK (phospho S910) antibody

• 产品名称

  Anti-FAK (phospho S910)抗体
  参阅全部 FAK 一抗

• 描述

  兔多克隆抗体to FAK (phospho S910)

• 宿主

  Rabbit

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat, Chicken, Xenopus laevis

• 免疫原

  Synthetic peptide corresponding to FAK (phospho S910). The sequence is conserved in human, mouse, rat, chicken and frog.

• 常规说明

  
  

  FAK is a non-receptor protein tyrosine kinase discovered as a substrate for Src and it is a key protein in integrin, growth factor and bioactive peptide signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

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• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7.30
  Preservative: 0.05% Sodium azide
  Constituents: PBS, 50% Glycerol, 0.1% BSA
  
  PBS is without Mg2+ and Ca2+ and BSA is IgG and protease free.

• 纯度

  Immunogen affinity purified

• 纯化说明

  Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Focal Adhesion Kinase enzyme and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phospho-serine, irrespective of the sequence. The final product is generated by affinity chromatography using a Focal Adhesion Kinase-derived peptide that is phosphorylated at serine 910.

• Primary antibody说明

  FAK is a non-receptor protein tyrosine kinase discovered as a substrate for Src and it is a key protein in integrin, growth factor and bioactive peptide signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab4794于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 396416 Chicken

• Entrez Gene: 5747 Human

• Entrez Gene: 14083 Mouse

• Entrez Gene: 25614 Rat

• Entrez Gene: 399286 Xenopus laevis

• Omim: 600758 Human

• SwissProt: Q00944 Chicken

• SwissProt: Q05397 Human

• SwissProt: P34152 Mouse

• SwissProt: O35346 Rat

• SwissProt: Q91738 Xenopus laevis

• Unigene: 395482 Human

• Unigene: 254494 Mouse

• Unigene: 2809 Rat

• Unigene: 6819 Xenopus laevis

别名

• FADK 1 antibody

• FADK antibody

• FAK related non kinase polypeptide antibody

• FAK1 antibody

• FAK1_HUMAN antibody

• Focal adhesion kinase 1 antibody

• Focal adhesion Kinase antibody

• Focal adhesion kinase isoform FAK Del33 antibody

• Focal adhesion kinase related nonkinase antibody

• FRNK antibody

• p125FAK antibody

• pp125FAK antibody

• PPP1R71 antibody

• Protein phosphatase 1 regulatory subunit 71 antibody

• Protein tyrosine kinase 2 antibody

• Protein-tyrosine kinase 2 antibody

• Ptk2 antibody

• PTK2 protein tyrosine kinase 2 antibody

• 

  Western blot - Anti-FAK (phospho S910) antibody (ab4794)

  Specificity for detecting FAK pS910 phosphorylation using a phospho-specific antibody (PSSA): Cell extracts prepared from mitotic human epithelial carcinoma cells expressing FAK were resolved by SDS-PAGE on a 10% Tris-glycine gel, and transferred to nitrocellulose. Membranes were incubated with 0.5 µg/mL ab4794, following prior incubation in the presence of the peptide immunogen (lane 1), a generic phosphoserine peptide (lane 2), the non-phosphopeptide corresponding to the FAK phosphopeptide (lane 3), or the absence of the peptide immunogen (lane 4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar detection method. The data show that only the phosphopeptide corresponding to the peptide immunogen blocks the antibody signal, thereby demonstrating the specificity of the ab4794 PSSA for the targeted phosphorylation site. Specificity for detecting FAK pS910 phosphorylatio