Anti-Claspin抗体
参阅全部 Claspin 一抗
兔多克隆抗体to Claspin
Rabbit
适用于: WB, IPmore details
与反应: Human
预测可用于: Chimpanzee, Gorilla
Synthetic peptide (Human) - which represents a portion of human Claspin encoded within exon 15.
HeLa cell lysate.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µl 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3721于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | 1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 150 kDa). | |
IP | Use at 2-10 µg/mg of lysate. |
Entrez Gene: 63967 Human
Omim: 605434 Human
SwissProt: Q9HAW4 Human
Unigene: 175613 Human
CLSPN antibody
CLSPN protein antibody
CLSPN_HUMAN antibody
hClaspin antibody
Hu Claspin antibody
Claspin antibody
claspin homolog (Xenopus laevis) antibody
Claspin homolog antibody
Immunoprecipitation - Anti-Claspin antibody (ab3721)
Claspin was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab3721 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab3721 at 0.1µg/mL.
Lane 1: ab3721 IP in HeLa whole cell lysate.
Lane 2: ab3721 IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds
Western blot - Anti-Claspin antibody (ab3721)
All lanes : Anti-Claspin antibody (ab3721) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK293T whole cell lysate at 50 µg
Predicted band size: 150 kDa
Exposure time: 30 seconds