Anti-Mucin 5AC antibody [45M1]

• 产品名称

  Anti-Mucin 5AC抗体[45M1]
  参阅全部 Mucin 5AC 一抗

• 描述

  小鼠单克隆抗体[45M1] to Mucin 5AC

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-Fr, mIHC, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  

• 免疫原

  Full length protein. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• IHC-P: Human, mouse, and rat stomach tissues; IHC-Fr: Mouse and rat stomach tissues; ICC/IF: A549 cells. mIHC: Human stomach tissue.

• 常规说明

  This product has switched from a hybridoma to recombinant production method on 8th March 2021.

  This product is a recombinant monoclonal antibody, which offers several advantages including:

  For more information see here.

• - High batch-to-batch consistency and reproducibility

• - Improved sensitivity and specificity

• - Long-term security of supply

• - Animal-free production

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)

• 浓度

• 批次浓度范围 100 µg 浓度为 0.998 - 1.02 mg/ml

• 1 mg 浓度为 0.2 mg/ml

• 20 µg 浓度为 0.2 mg/ml

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  45M1

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab3649于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

图片

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  Immunocytochemistry - Anti-Mucin 5AC antibody [45M1] (ab3649)This image is courtesy of an anonymous Abreview

  Immunocytochemistry analysis of paraformaldehyde-fixed 0.5% Triton in PBS-permeabilized human HBE cells staining with ab3649 at 1/300 dilution. Secondary antibody was Alexa Fluor® 594 Goat Thermo A-11005 at 1/250 dilution. Red: Actin Magenta: MUC5AC Blue: DNA. Samples were incubated with the primary antibody with 1% BSA in PBS for 16 hours at 4°C.

  See Abreview

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  Multiplex immunohistochemistry - Anti-Mucin 5AC antibody [45M1] (ab3649)

  Fluorescence multiplex immunohistochemical analysis of the human stomach (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-Mucin 5AC (ab3649, gray; Opal™690), anti-MUC-6 (ab223846, green; Opal™520) and anti-Rab3D (ab128997, red; Opal™570) on human stomach. Panel B: anti-MUC-6 stained on mucous neck cells. Panel C: anti-Mucin 5AC stained on surface mucous cells. Panel D: anti-Rab3D stained on Chief cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab3649 (1/5000 dilution), ab223846 (1/1000 dilution), and ab128997 (1/10000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

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  Immunocytochemistry/ Immunofluorescence - Anti-Mucin 5AC antibody [45M1] (ab3649)

  Immunocytochemistry analysis of A549 (human lung carcinoma cell line) cells labeling Mucin 5AC with ab3649 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-beta Tubulin rabbit monoclonal antibody (ab179513) at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

  Negative control 1: ab3649 (Mouse monoclonal antibody to Mucin 5AC at 1/100 dilution) and ab150080 (anti-Rabbit secondary Alexa Fluor® 594 at 1/1000 dilution)

  Negative control 2: ab179513 (Rabbit monoclonal antibody to beta Tubulin at 1/200 dilution) and ab150113 (anti-Mouse secondary Alexa Fluor® 488 at 1/1000 dilution)

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  Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

  Immunohistochemistry (Frozen sections) analysis of mouse stomach tissue labeling Mucin 5AC with ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• 

  Immunohistochemistry (Frozen sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

  Immunohistochemistry (Frozen sections) analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 1/100 dilution. Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113) was used as the secondary antibody at 1/1000 dilution (green). Nuclei counterstained with DAPI (blue).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labeling Mucin 5AC with ab3649 at 1/5000 dilution. Heat mediated antigen retrieval was performed using Citrate buffer pH 6 (epitope retrieval solution 1) for 20 minutes. The section was incubated with ab3649 for 30 mins at room temperature. Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mucin 5AC antibody [45M1] (ab3649)Image from Kikuchi M et al., BMC Gastroenterol. 2010 Jun 18;10:65. doi: 10.1186/1471-230X-10-65.; Fig 5.; 18 June 2010, BMC Gastroenterology 2010, 10:65

  This image was generated from the hybridoma version of the product.

  Immunohistochemical analysis of rat stomach tissue, staining Mucin 5AC with ab3649.
  
  Antigen retrieval was performed via heat mediation in a citrate buffer. Samples were blocked before incubating with primary antibody (1/100) for 1 hour at room temperature. An HRP-conjugated anti-mouse IgG was used as the secondary antibody and staining was detected using DAB.

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  Anti-Mucin 5AC antibody [45M1] (ab3649)