Anti-CAMKIV抗体
参阅全部 CAMKIV 一抗
兔多克隆抗体to CAMKIV
Rabbit
适用于: ICC, IHC-Fr, ICC/IF, IHC-P, WBmore details
与反应: Mouse, Rat, Dog, Human, Xenopus laevis, Non human primates, Plants, Turtle
Synthetic peptide corresponding to Mouse CAMKIV aa 127-143.
Sequence:
VEKGYYSERDAADAVKQ
(Peptide available as ab5859)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3557于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use a concentration of 2 µg/ml. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. PubMed: 18802105 | |
| IHC-P | 1/20 - 1/100. | |
| WB | (3) | Use a concentration of 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). This antibody detects an ~55 kDa protein representing CaMKIV in HeLa cell lysate. |
Entrez Gene: 814 Human
Entrez Gene: 12326 Mouse
Omim: 114080 Human
SwissProt: Q16566 Human
SwissProt: P08414 Mouse
Unigene: 591269 Human
Unigene: 222329 Mouse
Unigene: 11046 Rat
Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
Brain Ca++-calmodulin dependent protein kinase type IV antibody
Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
Calcium/calmodulin dependent protein kinase IV antibody
Calcium/calmodulin dependent protein kinase type IV antibody
Calcium/calmodulin-dependent protein kinase type IV antibody
CAM kinase 4 antibody
CAM kinase GR antibody
CAM kinase IV antibody
CAM kinase-GR antibody
CaMK 4 antibody
CAMK GR antibody
CaMK IV antibody
Camk4 antibody
CaMKGR antibody
IV antibody
KCC4_HUMAN antibody
MGC36771 antibody
Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody (ab3557)
Immunofluorescence analysis of CaM Kinase IV in SH-SY5Y cells using ab3557. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab3557 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by Alexa Fluor® 488 conjugate Goat anti-Rabbit IgG (H+L) at a 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody (ab3557)
Immunohistochemistry analysis of CaM Kinase IV using ab3557 at 1/100 dilution for 1 hour at 37ºC showing staining in the cytoplasm and nucleus of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left) followed by an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMKIV antibody (ab3557)
Immunohistochemistry analysis of CaM Kinase IV using ab3557 at 1/20 dilution for 1 hour at 37ºC showing staining in the cytoplasm and nucleus of paraffin-embedded rat brain tissue (right) compared to a negative control without primary antibody (left) followed by an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin. Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature.
Western blot - Anti-CAMKIV antibody (ab3557)
All lanes : Anti-CAMKIV antibody (ab3557) at 1 µg/ml
Lane 1 : Mouse Brain tissue extract
Lane 2 : Rat Brain tissue extract
Lane 3 : Rat Liver tissue extract
Lane 4 : Rat Kidney tissue extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/4000 dilution
Predicted band size: 52 kDa
Observed band size: 59 kDawhy is the actual band size different from the predicted?
A 59 kDa band corresponding to CaMKIV was observed in Mouse Brain, Rat Brain and not observed in other tissues which are documented to be CaMKIV negative.
Western blot - Anti-CAMKIV antibody (ab3557)
All lanes : Anti-CAMKIV antibody (ab3557) at 1 µg/ml
Lane 1 : PC12
Lane 2 : Rat Brain
Lane 3 : Mouse Brain
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Predicted band size: 52 kDa
A ~ 59 kDa band corresponding to CaM Kinase IV was observed across cell lines and tissues tested. Additionally ~25 kDa band was observed in PC12 cell line.
Blocking buffer: 5 % skimmed milk
