Anti-iNOS抗体
参阅全部 iNOS 一抗
兔多克隆抗体to iNOS
Rabbit
This antibody detects iNOS. It does not detect other NOS isoforms. By western blot, this antibody detects an ~135 kDa protein representing recombinant human iNOS. By western blot, this antibody also detects purified recombinant mouse iNOS, mouse iNOS from cytokine stimulated RAW 264.7 cells.
适用于: IHC-P, WB, ICC/IFmore details
与反应: Mouse, Human
Synthetic peptide corresponding to Mouse iNOS aa 1-100.
Database link: P29477
ICC: A549, NIH/3T3 cells. IHC-P: Human heart tissue.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
200 µl 浓度为 4 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3523于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (13) | 1/20. |
| WB | (15) | 1/200 - 1/1000. Predicted molecular weight: 131 kDa. |
| ICC/IF | (2) | 1/50. |
Entrez Gene: 4843 Human
Entrez Gene: 18126 Mouse
Omim: 163730 Human
SwissProt: P35228 Human
SwissProt: P29477 Mouse
Unigene: 709191 Human
Unigene: 2893 Mouse
inducible antibody
Inducible nitric oxide synthase antibody
Inducible NO synthase antibody
Inducible NOS antibody
iNOS antibody
MAC NOS antibody
Macrophage NOS antibody
Nitric oxide synthase 2 inducible antibody
Nitric oxide synthase 2 inducible macrophage antibody
nitric oxide synthase 2A (inducible, hepatocytes) antibody
Nitric oxide synthase antibody
Nitric oxide synthase inducible antibody
nitric oxide synthase, macrophage antibody
NOS 2 antibody
NOS antibody
Nos II antibody
NOS type II antibody
nos2 antibody
NOS2_HUMAN antibody
NOS2A antibody
NOS2A, Inducible, Hepatocyte antibody
Peptidyl-cysteine S-nitrosylase NOS2 antibody
HEP-NOS antibody
Hepatocyte NOS antibody
HEPNOS antibody
Western blot - Anti-iNOS antibody (ab3523)
All lanes : Anti-iNOS antibody (ab3523) at 1/1000 dilution
Lane 1 : RAW264 whole cell lysate untreated
Lane 2 : RAW264 whole cell lysate untreated stimulated with LPS at 1 µg/mL for 16 hours
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, HRP conjugate at 1/1000 dilution
Lane 2 : Goat anti-Rabbit IgG (Heavy Chain) HRP conjugate at 1/1000 dilution
Predicted band size: 131 kDa
Using 4-20% Tris-Glycine polyacrylamide gel and transferred to a nitrocellulose membrane, blocked with 5% Milk in TBST for at least 1 hour. The membrane was probed with ab3523 at 4°C overnight on a rocking platform, washed in TBST, and probed with the secondary antibody for 1 hour.
Western blot - Anti-iNOS antibody (ab3523)
All lanes : Anti-iNOS antibody (ab3523) at 1/1000 dilution
Lane 1 : Whole cell lysate of RAW 264.7
Lane 2 : Whole cell lysate of RAW 264.7 treated with LPS
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Predicted band size: 131 kDa
This was run using 4-12% Bis-Tris Protein Gel and a Nitrocellulose membrane.
Observed band at 130kDa in LPS treated RAW 264.7 cells, and an uncharacterized band at ~60kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iNOS antibody (ab3523)
Immunohistochemistry analysis of human heart tissue stained for iNOS without (negative control) or using ab3523 at 1/200 dilution overnight at 4°C in a humidified chamber, followed biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor.
To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature.
Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)
Immunofluorescence analysis of A549 (human lung carcinoma cell line) whole cells labelling iNOS (Left panel: green) without (control) or using ab3523 at 1/20 dilution overnight at 4°C, followed DyLight-488 conjugated secondary antibody. Counter stain: F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Cells were grown on chamber slides and fixed with formaldehyde prior to staining.
Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody (ab3523)
Immunofluorescence analysis of NIH/3T3 (mouse embryo fibroblast cell line) whole cells labelling iNOS (Left panel: green) without (control) or using ab3523 at 1/20 dilution overnight at 4°C, followed DyLight-488 conjugated secondary antibody. Counter stain: F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
Cells were grown on chamber slides and fixed with formaldehyde prior to staining.
