Anti-Aurora B antibody [mAbcam 3609]

• 产品名称

  Anti-Aurora B抗体[mAbcam 3609]
  参阅全部 Aurora B 一抗

• 描述

  小鼠单克隆抗体[mAbcam 3609] to Aurora B

• 宿主

  Mouse

• 特异性

  Based on the immunogen sequence, we do not expect the antibody to cross-react with AURKA or AURKC. No cross-reactivity testing has been performed.

• 经测试应用

  适用于: WB, IHC-Pmore details
  不适用于: ICC/IF

• 种属反应性

  与反应: Human
  预测可用于: Mouse, Rat

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 阳性对照

• Hela whole cell lysate

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: PBS

• 浓度

• 10 µg 浓度为 1 mg/ml

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  mAbcam 3609

• 同种型

  IgG1

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab3609于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Is unsuitable for ICC/IF.

数据库链接

• Entrez Gene: 9212 Human

• Entrez Gene: 20877 Mouse

• Entrez Gene: 114592 Rat

• Omim: 604970 Human

• SwissProt: Q96GD4 Human

• SwissProt: O70126 Mouse

• SwissProt: O55099 Rat

• Unigene: 442658 Human

• Unigene: 3488 Mouse

• Unigene: 10865 Rat

别名

• AIK2 antibody

• AIM-1 antibody

• AIM1 antibody

• ARK-2 antibody

• ARK2 antibody

• AurB antibody

• AURKB antibody

• AURKB_HUMAN antibody

• Aurora 1 antibody

• Aurora and Ipl1 like midbody associated protein 1 antibody

• Aurora kinase B antibody

• Aurora related kinase 2 antibody

• Aurora- and Ipl1-like midbody-associated protein 1 antibody

• Aurora-B antibody

• Aurora-related kinase 2 antibody

• Aurora/IPL1 related kinase 2 antibody

• Aurora/IPL1-related kinase 2 antibody

• IPL1 antibody

• PPP1R48 antibody

• Protein phosphatase 1 regulatory subunit 48 antibody

• Serine/theronine kinase 12 antibody

• Serine/threonine protein kinase 12 antibody

• Serine/threonine-protein kinase 12 antibody

• Serine/threonine-protein kinase aurora-B antibody

• STK-1 antibody

• STK1 antibody

• STK12 antibody

• STK5 antibody

• 

  Western blot - Anti-Aurora B antibody [mAbcam 3609] (ab3609)

  All lanes : Anti-Aurora B antibody [mAbcam 3609] (ab3609) at 1 µg/ml
  
  Lane 1 : Hek293 Whole Cell Lysate
  Lane 2 : Jurkat Whole Cell Lysate
  Lane 3 : HeLa Whole Cell Lysate
  Lane 4 : HeLa Whole Cell Lysate- Nocodozole Stimulated
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Absorbed (HRP) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 39 kDa
  Observed band size: 37 kDawhy is the actual band size different from the predicted?
  
  
  Exposure time: 3 minutes
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aurora B antibody [mAbcam 3609] (ab3609)Image courtesy of Human Protein Atlas

  Image courtesy of Human Protein Atlas

  Paraffin embedded human liver tumor tissue was incubated with ab3609 (1/35 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

  ab3609 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org