Anti-Dynamin 1抗体
参阅全部 Dynamin 1 一抗
兔多克隆抗体to Dynamin 1
Rabbit
适用于: WB, ICC/IFmore details
与反应: Rat, Human
Synthetic peptide corresponding to Rat Dynamin 1 aa 633-647.
Sequence:
KEKASETEENGSDSF
(Peptide available as ab4984)
WB: Rat homogenate. ICC/IF: SH-SY5Y cells.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µl 浓度为 1 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab3456于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa). | |
| ICC/IF | Use at an assay dependent concentration. |
Entrez Gene: 1759 Human
Omim: 602377 Human
SwissProt: Q05193 Human
Unigene: 522413 Human
Unigene: 10354 Rat
DNM antibody
DNM1 antibody
DYN1_HUMAN antibody
Dynamin antibody
Dynamin-1 antibody
Dynamin1 antibody
B dynamin antibody
D100 antibody
DNM 1 antibody
Western blot - Anti-Dynamin 1 antibody (ab3456)
Western blot of Dynamin 1 on rat brain homogenate using ab3456.
Immunocytochemistry/ Immunofluorescence - Anti-Dynamin 1 antibody (ab3456)
Immunofluorescence analysis of Dynamin I was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3456 at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
