Anti-STAT1 antibody [SM1]

• 产品名称

  Anti-STAT1抗体[SM1]
  参阅全部 STAT1 一抗

• 描述

  小鼠单克隆抗体[SM1] to STAT1

• 宿主

  Mouse

• 特异性

  The antibody recognizes an epitope included within amino acids 721-733 of the 91 kDa STAT 1 protein.

• 经测试应用

  适用于: WB, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Synthetic peptide:

  DNLLPMSPEEFDE

  , corresponding to amino acids 721-733 of STAT 1.

  Run BLAST with Run BLAST with 

• 阳性对照

• WB: HeLa, MCF-7. Cos-7 cell lysates. Flow Cyt: HeLa cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

• 克隆

  单克隆

• 克隆编号

  SM1

• 同种型

  IgG2b

The Abpromise guarantee

Abpromise™承诺保证使用ab3987于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 6772 Human

• Omim: 600555 Human

• SwissProt: P42224 Human

• Unigene: 642990 Human

• 别名

• IMD31A antibody

• IMD31B antibody

• IMD31C antibody

• ISGF 3 antibody

• ISGF-3 antibody

• OTTHUMP00000163552 antibody

• OTTHUMP00000165046 antibody

• OTTHUMP00000165047 antibody

• OTTHUMP00000205845 antibody

• Signal transducer and activator of transcription 1 91kDa antibody

• Signal transducer and activator of transcription 1 antibody

• Signal transducer and activator of transcription 1, 91kD antibody

• Signal transducer and activator of transcription 1-alpha/beta antibody

• STAT 1 antibody

• Stat1 antibody

• STAT1_HUMAN antibody

• STAT91 antibody

• Transcription factor ISGF 3 components p91 p84 antibody

• Transcription factor ISGF-3 components p91/p84 antibody

• Transcription factor ISGF3 components p91/p84 antibody

• XStat1 antibody

• Signal transducer and activator of transcription 1 91kD antibody

• CANDF7 antibody

• DKFZp686B04100 antibody

• 

  Western blot - Anti-STAT1 antibody [SM1] (ab3987)

  All lanes : Anti-STAT1 antibody [SM1] (ab3987) at 2 µg/ml
  
  Lane 1 : HeLa cell lysate (reducing conditions)
  Lane 2 : MCF-7 cell lysate (reducing conditions)
  Lane 3 : Cos-7 cell lysate (reducing conditions)
  
  Secondary
  All lanes : IRDye800-conjugated anti-mouse
  
  Predicted band size: 87 kDa
  Observed band size: 90 kDawhy is the actual band size different from the predicted?
  
  
  
  

  Western blotting analysis of STAT1 using ab3987 on lysates of HeLa, MCF-7, and Cos-7 cell lines under reducing conditions.

  Nitrocellulose membrane was probed with 2 µg/ml of ab3987 followed by IRDye800-conjugated anti-mouse secondary antibody. 

• 

  Flow Cytometry - Anti-STAT1 antibody [SM1] (ab3987)

  Overlay histogram showing HeLa cells stained with ab3987 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3987, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

• 

  Western blot - Anti-STAT1 antibody [SM1] (ab3987)

   Induction of phosphorylation of STAT 1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT-1 (Ser727) by the antibody PSM1 (upper panel) and total STAT 1 level by the antibody SM1 (lower panel).

  Induction of phosphorylation of STAT 1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT-1 (Ser727)