Anti-Rab3A抗体
参阅全部 Rab3A 一抗
兔多克隆抗体to Rab3A
Rabbit
适用于: WB, ICC/IFmore details
与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Human Rab3A aa 1-18.
Sequence:
MASATDSRYGQKESSDQN
(Peptide available as ab4951)
WB: mouse brain, kidney, liver, rat brain, kidney, liver; ICC: HeLa cells
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA
浓度
100 µg 浓度为 1 mg/ml
Protein A purified
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in co-ordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals and the best studied is rab 3A. Rab 3A is found to be abundant in presynaptic nerve terminals and is also found to be crucial in acrosomal exocytosis in human spermatozoa. Abnormal accumulation of rab 3A in the cytoplasm of Purkinje cells has been reported in the prion protein related Creutzfeldt-Jakob disease.
多克隆
IgG
Abpromise™承诺保证使用ab3335于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (7) | Use a concentration of 2 µg/ml. |
| ICC/IF | (1) | Use a concentration of 2 µg/ml. |
Entrez Gene: 5864 Human
Entrez Gene: 19339 Mouse
Omim: 179490 Human
SwissProt: P20336 Human
SwissProt: P63011 Mouse
Unigene: 27744 Human
Unigene: 5083 Mouse
Unigene: 44409 Rat
Rab 3A antibody
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RAB3A_HUMAN antibody
RAS associated protein RAB 3A antibody
RAS associated protein RAB3A antibody
Ras related protein Rab 3A antibody
Ras related protein Rab3A antibody
Ras-related protein Rab-3A antibody
Western blot - Anti-Rab3A antibody (ab3335)
All lanes : Left: ab3335 at 2 µg/mL.
Right: ab302518 at 1/1000 dilution
Lane 1 : Wild-type SK-N-FI cell lysate
Lane 2 : RAB3A knockout SK-N-FI cell lysate
Lane 3 : Human brain cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 27 kDawhy is the actual band size different from the predicted?
Western blot: Left: Anti-RAB3A antibody (ab3335) staining at 2 ug/ml. Right: Recombinant Anti-Rab3A antibody [EPR26022-9] (ab302518) staining at 1/1000 dilution. Anti-Rab3A antibodies shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab3335 and ab302518 were shown to bind specifically to RAB3A. A band was observed at 27 kDa in wild-type SK-N-FI cell lysates with no signal observed at this size in RAB3A knockout cell line. To generate this image, wild-type and RAB3A knockout SK-N-FI cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Rab3A antibody (ab3335)
Immunofluorescence analysis of RAB3A was done on 70% confluent log phase HeLa (Human epithelial adenocarcinoma cell line)
cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3335 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution. Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Western blot - Anti-Rab3A antibody (ab3335)
All lanes : Anti-Rab3A antibody (ab3335) at 2 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat kidney tissue lysate
Lane 6 : Rat liver tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
A 27 kDa band corresponding to RAB3A was observed along with two uncharacterized band (*) at ~40 kD and 60 kDa across tissues tested.
